Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,311,270 |
T→C |
V61A (GTT→GCT) |
tonB → |
Ton complex subunit TonB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,311,270 | 0 | T | C | 100.0%
| 85.0
/ NA
| 24 | V61A (GTT→GCT) | tonB | Ton complex subunit TonB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/11); total (13/11) |
CCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAG > NC_000913/1311203‑1311341
|
cctgcgcctgcgCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCg > 2:253911/1‑81 (MQ=255)
gcgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAg > 2:253921/1‑81 (MQ=255)
cgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGa < 1:253883/79‑1 (MQ=255)
gcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCggtg > 2:253919/1‑81 (MQ=255)
gcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCggtgg > 2:253920/1‑80 (MQ=255)
gATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGcc < 1:253884/81‑1 (MQ=255)
tttCTGTCACGATGGTTACGCCGGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAg < 2:253885/81‑1 (MQ=255)
tCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAAcc < 2:253886/79‑1 (MQ=255)
cACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCgg > 2:253925/1‑80 (MQ=255)
aCGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGaa > 1:253917/1‑81 (MQ=255)
cGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAc < 2:253887/81‑1 (MQ=255)
gATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAcc < 1:253888/81‑1 (MQ=255)
aTGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCt > 2:253918/1‑81 (MQ=255)
tGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTg < 2:253889/81‑1 (MQ=255)
gTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAg < 1:253890/81‑1 (MQ=255)
ctgATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGGGCCGATcccc > 2:253923/1‑80 (MQ=255)
gATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGaa > 1:253937/1‑81 (MQ=255)
gATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGaa > 1:253924/1‑81 (MQ=255)
ttctcGACCCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGaa < 1:253891/79‑1 (MQ=255)
tcGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGc > 1:253929/1‑81 (MQ=255)
gAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAAcc < 2:253892/77‑1 (MQ=255)
gAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGcc > 2:253922/1‑80 (MQ=255)
ccACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaa < 1:253893/81‑1 (MQ=255)
acaAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaag > 2:253926/1‑80 (MQ=255)
|
CCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAG > NC_000913/1311203‑1311341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
