Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,325,841 |
A→G |
T366A (ACG→GCG) |
yciQ → |
DUF2207 domain‑containing protein YciQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,325,841 | 0 | A | G | 100.0%
| 82.7
/ NA
| 24 | T366A (ACG→GCG) | yciQ | DUF2207 domain‑containing protein YciQ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/17); total (7/17) |
ATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTC > NC_000913/1325770‑1325917
|
atGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGAc < 2:236362/81‑1 (MQ=255)
cccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCt < 2:236363/79‑1 (MQ=255)
cccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTa < 1:236364/81‑1 (MQ=255)
ccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTAc < 2:236365/81‑1 (MQ=255)
gCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGc < 2:236366/81‑1 (MQ=255)
ttaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCAttttt < 1:236367/80‑1 (MQ=255)
ttaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCAttttt < 2:236368/80‑1 (MQ=255)
tGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGa > 2:236394/1‑81 (MQ=255)
gAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATg < 1:236369/81‑1 (MQ=255)
gCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAg < 2:236370/81‑1 (MQ=255)
ttATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGaaa > 1:236400/1‑81 (MQ=255)
ttATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGaaa > 1:236401/1‑81 (MQ=255)
tCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAtttt < 1:236371/80‑1 (MQ=255)
aaaGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTg > 1:236399/1‑81 (MQ=255)
aGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGcc > 1:236396/1‑81 (MQ=255)
ggCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcg < 2:236372/81‑1 (MQ=255)
gCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc < 1:236373/81‑1 (MQ=255)
gCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc < 2:236374/81‑1 (MQ=255)
cGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgcg < 2:236375/81‑1 (MQ=255)
gATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgcg > 1:236395/1‑80 (MQ=255)
gATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgcg > 1:236397/1‑80 (MQ=255)
tAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaa < 1:236376/81‑1 (MQ=255)
aGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaaaa < 2:236377/81‑1 (MQ=255)
cTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTc < 2:236378/80‑1 (MQ=255)
|
ATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTC > NC_000913/1325770‑1325917
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
