Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,370,044 |
(T)9→10 |
intergenic (+41/‑172) |
pspE → / → ycjM |
thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,370,035 | 1 | . | T | 100.0%
| 40.8
/ NA
| 13 | intergenic (+32/‑181) | pspE/ycjM | thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (4/9); total (4/9) |
AACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCT > NC_000913/1369956‑1370108
|
aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑t > 1:243689/1‑81 (MQ=255)
cGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑ttt < 2:243666/81‑1 (MQ=255)
gACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCacac < 1:243667/81‑1 (MQ=255)
gCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTAtt > 1:243695/1‑81 (MQ=255)
cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTAttt < 2:243668/81‑1 (MQ=255)
aTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc < 2:243669/81‑1 (MQ=255)
aTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc > 2:243687/1‑81 (MQ=255)
aCCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcac < 1:243670/81‑1 (MQ=255)
tGAGCGGCGCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt < 1:243671/81‑1 (MQ=255)
gcggcgCTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACg < 2:243672/81‑1 (MQ=255)
gcgcTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCtt < 1:243673/81‑1 (MQ=255)
gcgcTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCtt > 1:243693/1‑81 (MQ=255)
cgcTTATGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCttt < 2:243674/81‑1 (MQ=255)
aTGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCCCAAAAATACGCTTTTTccc > 2:243691/1‑81 (MQ=255)
tGCGCCGTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCt < 2:243675/81‑1 (MQ=255)
|
AACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCT > NC_000913/1369956‑1370108
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
