Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,433,224 |
C→T |
V151M (GTG→ATG) |
pinR ← |
Rac prophage; putative site‑specific recombinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,433,224 | 0 | C | T | 100.0%
| 47.5
/ NA
| 16 | V151M (GTG→ATG) | pinR | Rac prophage; putative site‑specific recombinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (3/13); total (3/13) |
CACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAA > NC_000913/1433161‑1433295
|
cACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAAc < 2:253654/81‑1 (MQ=255)
taCTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCAACCAAAACGTTTc < 1:253655/80‑1 (MQ=255)
cTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCt < 2:253656/81‑1 (MQ=255)
aCTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCggg < 1:253658/81‑1 (MQ=255)
aCTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCgg < 2:253657/80‑1 (MQ=255)
aaTTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGct < 1:253659/79‑1 (MQ=255)
gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATccc < 1:253663/81‑1 (MQ=255)
gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATccc < 2:253660/81‑1 (MQ=255)
gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATccc < 2:253662/81‑1 (MQ=255)
gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATcc < 2:253661/80‑1 (MQ=255)
caccTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAAt > 2:253680/1‑80 (MQ=255)
ttCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGt > 2:253679/1‑81 (MQ=18)
tCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGt < 1:253664/80‑1 (MQ=21)
tcttcATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAg > 2:253678/1‑80 (MQ=21)
cttcATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGt < 2:253665/80‑1 (MQ=21)
aTTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTaaaa < 2:253666/80‑1 (MQ=21)
|
CACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAA > NC_000913/1433161‑1433295
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
