Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,460,682 |
+T |
coding (212/423 nt) |
paaI → |
phenylacetyl‑CoA thioesterase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,460,682 | 1 | . | T | 100.0%
| 102.9
/ NA
| 31 | Q71L (CAG→CTG) | paaI | phenylacetyl‑CoA thioesterase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (23/8); total (23/8) |
GCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑GGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAA > NC_000913/1460602‑1460753
|
gCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCa > 2:258039/1‑81 (MQ=255)
aCGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑tgggg < 2:258004/81‑2 (MQ=255)
cGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑tggggc > 2:258026/1‑79 (MQ=255)
ggTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCt > 1:258036/1‑81 (MQ=255)
gTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTTCAATAGCCATGGGGCTg < 1:258005/81‑1 (MQ=255)
gTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTg > 2:258030/1‑81 (MQ=255)
atcaAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTgg > 2:258054/1‑79 (MQ=255)
tcaAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAg < 2:258006/81‑1 (MQ=255)
caAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGc > 1:258037/1‑81 (MQ=255)
aaaGTTGCCACGGCTGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGcc > 1:258072/1‑81 (MQ=255)
aaaGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGcc > 1:258038/1‑81 (MQ=255)
ccACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCtt < 2:258008/81‑1 (MQ=255)
ccACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCtt < 2:258007/81‑1 (MQ=255)
cggcggGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTctg > 1:258028/1‑81 (MQ=255)
ggCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTctgcctg > 1:258035/1‑80 (MQ=255)
ggCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTctgcctg > 1:258034/1‑80 (MQ=255)
aGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCAc > 1:258029/1‑80 (MQ=255)
tATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgatt > 2:258050/1‑81 (MQ=255)
tATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgatt > 2:258041/1‑81 (MQ=255)
aTTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgatt > 1:258033/1‑80 (MQ=255)
ttttCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgattga > 2:258052/1‑81 (MQ=255)
cTGGCTAATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGAtttttt < 1:258009/81‑1 (MQ=255)
gCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGc < 2:258010/80‑1 (MQ=255)
gATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTc > 2:258042/1‑80 (MQ=255)
tACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAg < 2:258011/81‑1 (MQ=255)
ccTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCttt < 2:258012/81‑1 (MQ=255)
tGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCg > 2:258043/1‑81 (MQ=255)
gCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCg > 2:258032/1‑80 (MQ=255)
gCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGacac > 1:258046/1‑81 (MQ=255)
ccTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGA‑TTTTTGCGTCCAGGCTTTGCCGGAGACACCt > 2:258049/1‑81 (MQ=255)
gCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCtt > 1:258051/1‑80 (MQ=255)
gCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCtt > 2:258057/1‑80 (MQ=255)
gCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTCTTTGCGTCCAGGCTTTGCCGGAGACACCtt > 2:258056/1‑80 (MQ=255)
cAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTaa > 1:258040/1‑81 (MQ=255)
|
GCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑GGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAA > NC_000913/1460602‑1460753
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
