Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,549,287 |
C→A |
S629S (TCC→TCA) |
fdnG → |
formate dehydrogenase N subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,549,287 | 0 | C | A | 97.1%
| 112.0
/ NA
| 34 | S629S (TCC→TCA) | fdnG | formate dehydrogenase N subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (19/14); total (20/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGCGGG > NC_000913/1549209‑1549364
|
tCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAgg > 2:272060/1‑81 (MQ=255)
cGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTc > 1:272049/1‑81 (MQ=255)
gCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGc < 1:272021/81‑1 (MQ=255)
cTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGt > 1:272080/1‑81 (MQ=255)
aTTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTg > 1:272065/1‑81 (MQ=255)
aaGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCAc < 1:272022/80‑1 (MQ=255)
tATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGa < 2:272023/81‑1 (MQ=255)
ttCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGaa < 2:272024/80‑1 (MQ=255)
tCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGt > 1:272062/1‑79 (MQ=255)
tGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAg > 2:272071/1‑80 (MQ=255)
tGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAg > 2:272052/1‑80 (MQ=255)
ccTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAc > 1:272066/1‑81 (MQ=255)
ccTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAc < 1:272025/81‑1 (MQ=255)
ttCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAcgc > 2:272064/1‑81 (MQ=255)
tCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAcgcg > 1:272057/1‑81 (MQ=255)
cTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGc < 1:272027/79‑1 (MQ=255)
cTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCg > 1:272061/1‑80 (MQ=255)
cTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCg > 1:272078/1‑80 (MQ=255)
cTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGa < 2:272026/81‑1 (MQ=255)
tGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGaa < 1:272028/79‑1 (MQ=255)
gCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAgc < 1:272029/80‑1 (MQ=255)
cTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAgcgc > 2:272063/1‑81 (MQ=255)
gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac > 1:272073/1‑81 (MQ=255)
gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac < 1:272030/81‑1 (MQ=255)
gaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAacgac < 2:272031/81‑1 (MQ=255)
aTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAacgacg > 1:272054/1‑81 (MQ=255)
gTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGt > 1:272072/1‑80 (MQ=255)
ctCGCTAACTCCCTTCTCTGTTTTCATTTGCACTGTACATCTCAGTACGCTCCTTGCGAATCGCGTAACGACGGTGAAAtt > 2:866035/2‑81 (MQ=255)
tCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTc < 1:272032/81‑1 (MQ=255)
cGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCt > 1:272081/1‑81 (MQ=255)
tAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGc > 1:272070/1‑81 (MQ=255)
aCTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGc < 2:272033/79‑1 (MQ=255)
cTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGCggg > 1:272090/1‑81 (MQ=255)
cTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGCggg < 2:272034/81‑1 (MQ=255)
|
TCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGCGGG > NC_000913/1549209‑1549364
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
