Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,549,287 |
C→A |
S629S (TCC→TCA) |
fdnG → |
formate dehydrogenase N subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,549,287 | 0 | C | A | 100.0%
| 107.3
/ NA
| 30 | S629S (TCC→TCA) | fdnG | formate dehydrogenase N subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/16); total (14/16) |
TCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTG > NC_000913/1549209‑1549359
|
tCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAgg > 2:292721/1‑81 (MQ=255)
ccGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGt > 2:292703/1‑81 (MQ=255)
cGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGt < 1:292675/80‑1 (MQ=255)
cGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTc < 2:292676/81‑1 (MQ=255)
gCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGc < 1:292677/81‑1 (MQ=255)
tCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTgg > 1:292720/1‑81 (MQ=255)
tCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTg > 2:292728/1‑79 (MQ=255)
tCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTg > 2:292711/1‑79 (MQ=255)
aGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGt > 2:292715/1‑81 (MQ=255)
cTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGc < 1:292678/81‑1 (MQ=255)
aGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTg > 2:292716/1‑81 (MQ=255)
gCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGa > 2:292713/1‑81 (MQ=255)
ccTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAc < 2:292679/81‑1 (MQ=255)
cTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAcg < 1:292680/81‑1 (MQ=255)
cTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGa < 2:292681/81‑1 (MQ=255)
tttGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGcc > 2:292723/1‑78 (MQ=255)
tGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGaa < 1:292682/79‑1 (MQ=255)
aagaagATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGcgcg < 2:292683/79‑1 (MQ=255)
aagaagATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGt > 1:292717/1‑80 (MQ=255)
aagaagATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGt > 1:292710/1‑80 (MQ=255)
agaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTaa < 2:292684/81‑1 (MQ=255)
gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac < 2:292685/81‑1 (MQ=255)
gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac < 2:292686/81‑1 (MQ=255)
gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac < 1:292687/81‑1 (MQ=255)
agaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAacga > 1:292718/1‑81 (MQ=255)
gTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGt > 1:292719/1‑80 (MQ=255)
cTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGaaa > 1:292732/1‑81 (MQ=255)
tCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGaaa < 1:292688/78‑1 (MQ=255)
tCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAAt < 2:292689/79‑1 (MQ=255)
gCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTg < 1:292690/81‑1 (MQ=255)
|
TCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTG > NC_000913/1549209‑1549359
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
