Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,567,095 |
+C |
coding (46/1383 nt) |
dosC ← |
diguanylate cyclase DosC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,567,094 | 1 | . | C | 100.0%
| 69.8
/ NA
| 20 | V16G (GTC→GGC) | dosC | diguanylate cyclase DosC |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (5/15); total (5/15) |
ACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGA‑CAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGC > NC_000913/1567017‑1567160
|
aCTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGA‑cca < 2:275125/81‑3 (MQ=255)
gATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTc > 1:275157/1‑81 (MQ=255)
tACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCg < 1:275126/81‑1 (MQ=255)
aGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTcc < 2:275127/80‑1 (MQ=255)
tAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTc < 2:275128/81‑1 (MQ=255)
aTGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCAt < 1:275129/81‑1 (MQ=255)
aGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCtt < 1:275130/81‑1 (MQ=255)
cATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTc < 2:275131/81‑1 (MQ=255)
aTGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCa > 1:275155/1‑81 (MQ=255)
aaCGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCttt > 1:275153/1‑81 (MQ=255)
ggCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTaa < 1:275132/81‑1 (MQ=255)
tttCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATAc < 1:275133/81‑1 (MQ=255)
ttCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACa > 1:275156/1‑81 (MQ=255)
tCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACa < 1:275134/80‑1 (MQ=255)
ccGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACAt < 1:275135/80‑1 (MQ=255)
cgGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTcc < 1:275136/81‑1 (MQ=255)
cTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCAt > 2:275158/1‑80 (MQ=255)
cACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTcac < 1:275137/81‑1 (MQ=255)
cGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCacac < 1:275138/81‑1 (MQ=255)
gAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACAcc < 2:275139/81‑1 (MQ=255)
ccTGTTCCACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGc < 2:275140/76‑1 (MQ=255)
|
ACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGA‑CAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGC > NC_000913/1567017‑1567160
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
