Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,728,574 |
C→T |
A76A (GCC→GCT) |
rnt → |
RNase T |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,728,574 | 0 | C | T | 100.0%
| 78.9
/ NA
| 23 | A76A (GCC→GCT) | rnt | RNase T |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/17); total (6/17) |
GGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGA > NC_000913/1728498‑1728639
|
ggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTc > 1:298158/1‑80 (MQ=255)
gctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCaa > 2:298153/1‑81 (MQ=255)
ctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAAc < 1:298120/81‑1 (MQ=255)
tGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCa < 2:298121/81‑1 (MQ=255)
ccGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCAtt < 2:298122/81‑1 (MQ=255)
cGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTg < 2:298123/81‑1 (MQ=255)
ggACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGa > 1:298154/1‑81 (MQ=255)
cacaTTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGaa < 1:298124/81‑1 (MQ=255)
acattacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAAc < 2:298125/81‑1 (MQ=255)
attacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGa < 1:298126/81‑1 (MQ=255)
cACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATcg < 1:298127/80‑1 (MQ=255)
aCGTCGAACCATTTGTCGGCGCAAATTTGCAGCCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATcgcg < 2:298128/81‑1 (MQ=255)
ccATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTc < 2:298129/81‑1 (MQ=255)
aTTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAg < 1:298130/81‑1 (MQ=255)
tttGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGc < 1:298131/81‑1 (MQ=255)
tGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCg < 2:298132/80‑1 (MQ=255)
cgcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGa < 1:298133/81‑1 (MQ=255)
cAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAgg < 2:298134/81‑1 (MQ=255)
tttGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCt < 2:298135/81‑1 (MQ=255)
ttGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTg > 1:298161/1‑81 (MQ=255)
tGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTg > 1:298160/1‑80 (MQ=255)
gCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCa > 1:298155/1‑81 (MQ=255)
aCCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGa < 2:298136/81‑1 (MQ=255)
|
GGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGA > NC_000913/1728498‑1728639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
