Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,728,574 |
C→T |
A76A (GCC→GCT) |
rnt → |
RNase T |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,728,574 | 0 | C | T | 100.0%
| 56.6
/ NA
| 17 | A76A (GCC→GCT) | rnt | RNase T |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/9); total (8/9) |
CTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTT > NC_000913/1728496‑1728645
|
ctggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCttt < 1:319756/81‑1 (MQ=255)
ggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTc > 2:319791/1‑80 (MQ=255)
tgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACg > 1:585919/1‑81 (MQ=255)
gATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCATATTTGCAACCAGAAGCCCTCGCTTTCAACgg > 2:319785/1‑81 (MQ=255)
aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAATTTTGCAACCAGAAGCCCTCGCTTTCAACgg > 2:319787/1‑80 (MQ=255)
cGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCAtt > 1:319790/1‑80 (MQ=255)
acacCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGAcc < 2:319757/81‑1 (MQ=255)
cacaTTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGaa < 2:319758/81‑1 (MQ=255)
acattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATc < 1:319759/80‑1 (MQ=255)
cACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATcgc < 1:319760/81‑1 (MQ=255)
aCGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATcgcg > 1:319794/1‑81 (MQ=255)
gcgcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACg < 1:319761/81‑1 (MQ=255)
gcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAg < 2:319763/81‑1 (MQ=255)
gcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAg < 1:319762/81‑1 (MQ=255)
aaTTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGgcg > 2:319793/1‑81 (MQ=255)
aTTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGgcgc < 1:319764/81‑1 (MQ=255)
aaCCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCAc > 2:319795/1‑80 (MQ=255)
aGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAAtttt < 2:319765/81‑1 (MQ=255)
|
CTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTT > NC_000913/1728496‑1728645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
