Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,730,699 |
C→T |
A538V (GCT→GTT) |
lhr → |
putative ATP‑dependent helicase Lhr |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,730,699 | 0 | C | T | 100.0%
| 107.0
/ NA
| 31 | A538V (GCT→GTT) | lhr | putative ATP‑dependent helicase Lhr |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/13); total (18/13) |
CTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGCTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTG > NC_000913/1730622‑1730773
|
ctctGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTc > 2:298543/1‑80 (MQ=255)
tGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAAtt > 2:298553/1‑81 (MQ=255)
ggCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAAttg > 1:298557/1‑81 (MQ=255)
gCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAAttgt < 1:298519/81‑1 (MQ=255)
tttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCgg > 1:298559/1‑81 (MQ=255)
cTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTAc > 1:298544/1‑79 (MQ=255)
tttCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgc > 2:298551/1‑81 (MQ=255)
ttCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAg < 1:508053/79‑1 (MQ=255)
tCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcgg > 1:298550/1‑81 (MQ=255)
cGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc > 1:298547/1‑81 (MQ=255)
cccAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCggcggc < 1:298520/81‑1 (MQ=255)
aaCTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCa > 1:298606/1‑81 (MQ=255)
aCTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCAt < 2:298521/81‑1 (MQ=255)
cTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCAtt > 2:298566/1‑81 (MQ=255)
ggTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTcc > 2:298548/1‑81 (MQ=255)
gTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTcc < 2:298522/80‑1 (MQ=255)
gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGAt < 2:298524/79‑1 (MQ=255)
gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATc < 2:298523/80‑1 (MQ=255)
gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCg > 2:298545/1‑81 (MQ=255)
aaCAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGt < 2:298525/81‑1 (MQ=255)
aCAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTg < 2:298526/81‑1 (MQ=255)
cAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTgg < 2:298527/81‑1 (MQ=255)
aCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtat < 1:298528/81‑1 (MQ=255)
tGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGttat > 1:298549/1‑81 (MQ=255)
ccGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTAc > 1:298563/1‑81 (MQ=255)
gCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTAccc > 1:298558/1‑81 (MQ=255)
gCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTAccc > 2:298556/1‑81 (MQ=255)
gACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGCAg > 2:298561/1‑81 (MQ=255)
gACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAg < 1:298529/81‑1 (MQ=255)
ccTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGt > 2:298552/1‑81 (MQ=255)
cTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTg < 2:298530/81‑1 (MQ=255)
|
CTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGCTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTG > NC_000913/1730622‑1730773
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
