Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,730,699 |
C→T |
A538V (GCT→GTT) |
lhr → |
putative ATP‑dependent helicase Lhr |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,730,699 | 0 | C | T | 100.0%
| 100.3
/ NA
| 28 | A538V (GCT→GTT) | lhr | putative ATP‑dependent helicase Lhr |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/20); total (8/20) |
CCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGCTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAG > NC_000913/1730621‑1730776
|
ccTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTc < 1:320161/81‑1 (MQ=255)
gCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAAtt < 1:320162/79‑1 (MQ=255)
aTTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTgg > 2:320201/1‑81 (MQ=255)
aTTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTgg > 1:320207/1‑81 (MQ=255)
tttttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGc < 2:320164/81‑1 (MQ=255)
tttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCgg > 1:320202/1‑81 (MQ=255)
ttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCgg < 2:320165/80‑1 (MQ=255)
tCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGt > 1:320208/1‑80 (MQ=255)
ttCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgc < 1:320166/80‑1 (MQ=255)
gCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc < 1:921047/80‑1 (MQ=255)
gCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc < 2:320168/80‑1 (MQ=255)
gCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc < 1:320167/80‑1 (MQ=255)
cAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCAc < 2:320169/81‑1 (MQ=255)
tCTGGAACAGGGAGACCGGGATATTGACCGCCCGATCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCgg < 2:320171/81‑1 (MQ=255)
tCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCgg < 2:320170/81‑1 (MQ=255)
gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATc < 2:320173/80‑1 (MQ=255)
gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATc < 2:320172/80‑1 (MQ=255)
aCAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTg < 1:320174/81‑1 (MQ=255)
cAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTgg > 1:320214/1‑81 (MQ=255)
gagaCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATg > 2:320218/1‑81 (MQ=255)
agaCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATg > 1:320210/1‑80 (MQ=255)
aCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtat < 2:320175/81‑1 (MQ=255)
aCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtat < 2:320176/81‑1 (MQ=255)
ccGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtata < 1:320177/81‑1 (MQ=255)
atatTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCgtg < 1:320178/81‑1 (MQ=255)
gCCCGACCTGGCGTTCAATTGTTGTCGGTTACCAACATCGGCACCATTCCTGATCGTGTCATGTATAGCGTGTTATTAccc < 2:320179/81‑1 (MQ=255)
cGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGaa < 1:320180/81‑1 (MQ=255)
ggCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTg > 2:320216/1‑79 (MQ=255)
gcgtTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTgaag < 1:320181/81‑1 (MQ=255)
|
CCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGCTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAG > NC_000913/1730621‑1730776
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
