Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,822,440 |
Δ1 bp |
intergenic (‑184/‑18) |
osmE ← / → nadE |
osmotically‑inducible lipoprotein OsmE/NAD synthetase, NH3‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,822,437 | 0 | T | . | 100.0%
| 97.2
/ NA
| 22 | intergenic (‑181/‑21) | osmE/nadE | osmotically‑inducible lipoprotein OsmE/NAD synthetase, NH3‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (9/13); total (9/13) |
TGACGTTTTAACCACCATTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGAT > NC_000913/1822360‑1822507
|
tGACGTTTTAACCACCATTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCtttc > 2:312032/1‑80 (MQ=255)
tAACCACCATTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTtctgtctg > 2:312043/1‑79 (MQ=255)
tAACCACCATTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTgg < 2:312013/80‑1 (MQ=255)
accaccATTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGGTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAgg < 2:312014/81‑1 (MQ=255)
caTTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGtt > 2:312046/1‑80 (MQ=255)
caTTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTc < 1:312017/81‑1 (MQ=255)
caTTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTc < 2:312015/81‑1 (MQ=255)
caTTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTc < 2:312016/81‑1 (MQ=255)
cGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACAtt < 2:312018/81‑1 (MQ=255)
gTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGcaa > 2:312045/1‑81 (MQ=255)
tCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGcaac < 1:312019/81‑1 (MQ=255)
tCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGcaac < 2:312022/81‑1 (MQ=255)
tCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGcaac < 2:312021/81‑1 (MQ=255)
tCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGcaa < 1:312020/80‑1 (MQ=255)
tCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAataata > 2:312047/1‑80 (MQ=255)
gCAACGGGTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGgcgc < 1:312023/81‑1 (MQ=255)
ggTTAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGgcg < 2:312024/81‑1 (MQ=255)
tAGCTTTAAGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCa > 1:312030/1‑80 (MQ=255)
aaGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCg > 1:312050/1‑79 (MQ=255)
aaGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCg > 2:312053/1‑79 (MQ=255)
aaGGAAGTTTTGTC‑TTTCTGTCTGGAGGGGATCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGc < 2:312025/80‑1 (MQ=255)
ggAAGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCg > 1:312048/1‑77 (MQ=255)
aaGTTTTGTC‑TTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGAt > 1:312057/1‑80 (MQ=255)
|
TGACGTTTTAACCACCATTCAGCCCGCTGTCGCTTGTCGTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGAT > NC_000913/1822360‑1822507
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
