Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,831,779 |
C→T |
A68A (GCG→GCA) |
astC ← |
succinylornithine transaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,831,779 | 0 | C | T | 100.0%
| 116.4
/ NA
| 32 | A68A (GCG→GCA) | astC | succinylornithine transaminase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/17); total (15/17) |
GCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTC > NC_000913/1831705‑1831856
|
gCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgt < 2:336339/81‑1 (MQ=255)
gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt > 1:336375/1‑81 (MQ=255)
gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt < 2:336340/81‑1 (MQ=255)
gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt > 2:336368/1‑81 (MQ=255)
gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt > 2:336385/1‑81 (MQ=255)
gCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACg < 2:336341/80‑1 (MQ=255)
cAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAg > 2:336382/1‑81 (MQ=255)
gaaGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTcc < 2:336343/78‑1 (MQ=255)
gCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTcc < 2:336342/80‑1 (MQ=255)
gCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCg > 2:336398/1‑81 (MQ=255)
ccGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATgc < 1:336344/80‑1 (MQ=255)
ggCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCa > 1:336380/1‑81 (MQ=255)
gTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATggtc > 2:336388/1‑79 (MQ=255)
ttGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGccc < 2:336345/81‑1 (MQ=255)
ggTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCa < 2:336346/80‑1 (MQ=255)
ggTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAg > 2:336391/1‑81 (MQ=255)
gtgtAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAgc < 1:336347/81‑1 (MQ=255)
gtAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAgcgc > 2:336384/1‑81 (MQ=255)
aCCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGtt < 1:336348/81‑1 (MQ=255)
ccGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTc < 2:336349/81‑1 (MQ=255)
gTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCAc < 1:336350/81‑1 (MQ=255)
tGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCg > 2:336386/1‑81 (MQ=255)
ccGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCa < 1:336351/81‑1 (MQ=255)
ggTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCTCAGCGCGTTCACCGCAAt > 1:336393/1‑81 (MQ=255)
ggTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAAt > 1:336383/1‑81 (MQ=255)
tATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc > 1:336396/1‑81 (MQ=255)
tATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc < 2:336352/81‑1 (MQ=255)
tATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc < 1:336353/81‑1 (MQ=255)
gCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCAc > 2:336399/1‑81 (MQ=255)
aaCTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCcgcg < 2:336354/81‑1 (MQ=255)
acttacttGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCcgcg > 1:336397/1‑80 (MQ=255)
acttacttGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGa < 1:336355/81‑1 (MQ=255)
aCTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGt < 1:336356/80‑1 (MQ=255)
aCTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTc > 1:336387/1‑81 (MQ=255)
|
GCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTC > NC_000913/1831705‑1831856
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
