Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,915,145 |
C→A |
E130D (GAG→GAT) |
proQ ← |
RNA chaperone ProQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,915,145 | 0 | C | A | 100.0%
| 88.3
/ NA
| 26 | E130D (GAG→GAT) | proQ | RNA chaperone ProQ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/17); total (9/17) |
GTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCG > NC_000913/1915069‑1915218
|
gTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCAc < 2:326839/81‑1 (MQ=255)
tACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACcag > 2:326896/1‑81 (MQ=255)
cGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACcagca < 2:326840/81‑1 (MQ=255)
aGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGc > 1:326891/1‑81 (MQ=255)
gcgcCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCg < 2:326841/81‑1 (MQ=255)
gcCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGc < 1:326842/81‑1 (MQ=255)
ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtt < 1:326843/81‑1 (MQ=255)
ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtt > 2:326888/1‑81 (MQ=255)
gcatggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGttt < 2:326844/78‑1 (MQ=255)
gtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGttttt > 1:326895/1‑81 (MQ=255)
tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtttttt < 1:326845/81‑1 (MQ=255)
tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtttttt > 1:326893/1‑81 (MQ=255)
gTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTg < 2:326846/80‑1 (MQ=255)
tCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCt < 2:326847/81‑1 (MQ=255)
gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGtt < 2:326848/81‑1 (MQ=255)
gCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg > 1:326892/1‑81 (MQ=255)
cgcggcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGTAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc < 1:390582/80‑1 (MQ=255)
cgcggcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc < 1:326849/80‑1 (MQ=255)
cgcggcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCt > 2:326907/1‑81 (MQ=255)
gcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAac < 2:326850/81‑1 (MQ=255)
cggTGCGTCTTCTTTATCACCAGCAGTTGCGACAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcg < 2:326851/81‑1 (MQ=255)
tGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACgcgc > 2:326906/1‑81 (MQ=255)
cGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt > 2:326897/1‑81 (MQ=255)
gTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCttt < 2:326852/81‑1 (MQ=255)
tcttctTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc < 1:326853/81‑1 (MQ=255)
tcttctTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc < 1:326854/81‑1 (MQ=255)
ctttatCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg < 2:326855/78‑1 (MQ=255)
|
GTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCG > NC_000913/1915069‑1915218
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
