Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,952,029 |
T→C |
L212S (TTA→TCA) |
yecE → |
DUF72 domain‑containing protein YecE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,952,029 | 0 | T | C | 100.0%
| 71.5
/ NA
| 21 | L212S (TTA→TCA) | yecE | DUF72 domain‑containing protein YecE |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/11); total (10/11) |
ATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGA > NC_000913/1951957‑1952104
|
aTGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGACGATATGACGCAAAACCGGGAATCATTTCAgg > 2:333261/1‑81 (MQ=255)
cTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCt < 1:333232/81‑1 (MQ=255)
tGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTg > 1:333263/1‑81 (MQ=255)
cGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACaaa < 2:333233/81‑1 (MQ=255)
gCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACaaaaa > 1:333266/1‑81 (MQ=255)
cAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAg < 1:333234/81‑1 (MQ=255)
cGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAg < 2:333235/80‑1 (MQ=255)
cTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTgg > 2:333273/1‑81 (MQ=255)
tCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATc < 1:333236/81‑1 (MQ=255)
ttATCGGTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCa > 2:333260/1‑81 (MQ=255)
ggTAGTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACg < 2:333237/81‑1 (MQ=255)
gTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCtt < 1:333238/81‑1 (MQ=255)
gTGATGATATGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCtt < 2:333239/81‑1 (MQ=255)
atGACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCttttt > 2:333268/1‑81 (MQ=255)
gACGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCtttttt > 2:333267/1‑80 (MQ=255)
aCGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCttttttt < 1:333240/80‑1 (MQ=255)
aCGCAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTa > 2:333272/1‑81 (MQ=255)
cAAAACCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACAt < 1:333241/81‑1 (MQ=255)
aaaCCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATAc > 1:333271/1‑81 (MQ=255)
aaaCCGGGAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATAc < 2:333242/81‑1 (MQ=255)
gAATCATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGa > 2:333275/1‑80 (MQ=255)
|
ATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGA > NC_000913/1951957‑1952104
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
