Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,153,777 |
T→C |
noncoding (132/136 nt) |
sibB → |
small RNA SibB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,153,777 | 0 | T | C | 100.0%
| 122.3
/ NA
| 35 | noncoding (132/136 nt) | sibB | small RNA SibB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/14); total (21/14) |
GACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAG > NC_000913/2153702‑2153853
|
gACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTccc > 1:365906/1‑81 (MQ=255)
cTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGt > 1:365910/1‑81 (MQ=255)
aaGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGttt > 2:365909/1‑81 (MQ=255)
acGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTccc < 1:365874/81‑1 (MQ=255)
tgatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTg < 1:365875/81‑1 (MQ=255)
tgatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTg < 2:365876/81‑1 (MQ=255)
tAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgt > 1:365914/1‑81 (MQ=255)
aGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc > 1:365913/1‑81 (MQ=255)
aGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc > 2:365917/1‑81 (MQ=255)
atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt < 2:365880/80‑1 (MQ=255)
atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt < 1:365878/80‑1 (MQ=255)
atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt < 2:365877/80‑1 (MQ=255)
atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt < 2:365879/80‑1 (MQ=255)
atGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcct > 2:365919/1‑81 (MQ=255)
tGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcctc > 1:365915/1‑81 (MQ=255)
gACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcctcc > 2:365920/1‑81 (MQ=255)
ccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCttt > 1:365923/1‑81 (MQ=255)
cTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTc < 1:365881/81‑1 (MQ=255)
ttCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGc > 2:365907/1‑81 (MQ=255)
cTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCAt < 1:365882/81‑1 (MQ=255)
tatttatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATg > 2:365922/1‑80 (MQ=255)
tatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg > 2:365908/1‑80 (MQ=255)
atGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg > 1:365924/1‑79 (MQ=255)
ccccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt < 1:365883/81‑1 (MQ=255)
cccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTa < 1:365884/81‑1 (MQ=255)
ccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAt < 1:365885/81‑1 (MQ=255)
ttCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCg > 2:365932/1‑81 (MQ=255)
ttCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCg > 1:365925/1‑81 (MQ=255)
cTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCt > 1:365916/1‑80 (MQ=255)
cGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc > 2:365931/1‑81 (MQ=255)
cGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc > 1:365911/1‑81 (MQ=255)
gggAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc < 2:365886/80‑1 (MQ=255)
ggAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcgc > 2:365912/1‑81 (MQ=255)
gggCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAg < 2:365887/81‑1 (MQ=255)
ggCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcgcg > 2:365918/1‑77 (MQ=255)
|
GACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAG > NC_000913/2153702‑2153853
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
