Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,259,563 |
T→C |
intergenic (‑267/+156) |
psuK ← / ← fruA |
putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,259,563 | 0 | T | C | 100.0%
| 60.6
/ NA
| 18 | intergenic (‑267/+156) | psuK/fruA | putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/11); total (7/11) |
GAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTA > NC_000913/2259487‑2259639
|
gAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTCGAg > 1:384185/1‑80 (MQ=255)
aTTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGaa < 1:384166/81‑1 (MQ=255)
aCAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAAt > 2:384183/1‑80 (MQ=255)
gAGGAATTTGTGACGGGATGCAGAGAACAGAAGAATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAgt < 1:384167/81‑1 (MQ=255)
aCGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAAc < 2:384168/80‑1 (MQ=255)
aTGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGa < 2:384169/81‑1 (MQ=255)
gaacagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCt < 2:384170/81‑1 (MQ=255)
agaacaATAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACt > 2:384186/1‑80 (MQ=255)
aaTAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGa < 2:384171/81‑1 (MQ=255)
tAAAAAAGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGAt > 2:384189/1‑80 (MQ=255)
aaaGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAc < 1:384172/81‑1 (MQ=255)
aaaGGGCAGAAAATAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAc > 2:384188/1‑81 (MQ=255)
aaaaTAATCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAt > 1:384187/1‑81 (MQ=255)
atCTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa < 1:384173/80‑1 (MQ=255)
cTGCCCTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaaa > 1:384199/1‑81 (MQ=255)
cccTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaa < 2:384174/77‑1 (MQ=255)
cccTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAAtt < 1:384175/80‑1 (MQ=255)
cccTCGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTa < 2:384176/81‑1 (MQ=255)
|
GAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTA > NC_000913/2259487‑2259639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
