Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,264,101 |
A→T |
D80V (GAT→GTT) |
setB → |
sugar exporter SetB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,264,101 | 0 | A | T | 100.0%
| 90.7
/ NA
| 26 | D80V (GAT→GTT) | setB | sugar exporter SetB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (9/17); total (9/17) |
GATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAACTAC > NC_000913/2264023‑2264177
|
gATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTc < 2:385162/81‑1 (MQ=255)
gATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTc < 2:385161/81‑1 (MQ=255)
ttcttcTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGc < 1:385163/81‑1 (MQ=255)
cttcttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCa < 1:385164/80‑1 (MQ=255)
ttcttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCaaa > 2:385197/1‑81 (MQ=255)
cttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCaa < 1:385165/78‑1 (MQ=255)
cttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCaa < 2:385166/78‑1 (MQ=255)
cGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGAt < 1:385167/81‑1 (MQ=255)
ggCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGAtt < 1:385168/81‑1 (MQ=255)
ggCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGAtt > 1:385200/1‑81 (MQ=255)
cAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGt > 1:385204/1‑81 (MQ=255)
tCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTtgct < 2:385169/81‑1 (MQ=255)
ggATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGt > 1:385202/1‑81 (MQ=255)
gATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGtt < 1:385171/81‑1 (MQ=255)
gATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGtt < 2:385170/81‑1 (MQ=255)
gTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGt < 1:385172/80‑1 (MQ=255)
ctcGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCAc < 2:385173/80‑1 (MQ=255)
gCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCAcc < 2:385175/78‑1 (MQ=255)
gCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCAcc < 2:385174/78‑1 (MQ=255)
gggCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCttttt > 2:385205/1‑81 (MQ=255)
cgcTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGcc < 2:385176/81‑1 (MQ=255)
tGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGaa > 1:385210/1‑81 (MQ=255)
aaGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCg > 1:385206/1‑80 (MQ=255)
aGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCa < 1:385177/81‑1 (MQ=255)
cggcgTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAacta > 2:825073/1‑81 (MQ=255)
ggcgTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAactac > 1:385213/1‑81 (MQ=255)
|
GATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAACTAC > NC_000913/2264023‑2264177
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
