Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,371,842 |
T→A |
L312* (TTG→TAG) |
arnT → |
lipid IVA 4‑amino‑4‑deoxy‑L‑arabinosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,371,842 | 0 | T | A | 100.0%
| 78.3
/ NA
| 23 | L312* (TTG→TAG) | arnT | lipid IVA 4‑amino‑4‑deoxy‑L‑arabinosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (12/11); total (12/11) |
GCAAGCATTCCGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATTGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCTGGCAGCAAAAAATA > NC_000913/2371764‑2371919
|
gCAAGCATTCCGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGc > 2:404413/1‑81 (MQ=255)
cAAGCATTCCGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGcc > 2:404417/1‑81 (MQ=255)
ttCCGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCta > 2:404425/1‑81 (MQ=255)
ccGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCtat < 2:404390/80‑1 (MQ=255)
ccGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCtat < 2:404391/80‑1 (MQ=255)
cGCAACCGTCTATTTGTTGAGCTAGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCtata < 1:404392/80‑1 (MQ=255)
cAACCGTCTATTTGTTGAGCTGGACGTTAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTc > 2:404421/1‑81 (MQ=255)
cAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTc < 1:404393/81‑1 (MQ=255)
cAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTc > 2:404415/1‑81 (MQ=255)
aaCCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCt > 1:404420/1‑81 (MQ=255)
cGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTc < 2:404394/81‑1 (MQ=255)
gACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTgg > 1:404422/1‑80 (MQ=255)
gACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGc < 1:404395/81‑1 (MQ=255)
gACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGc < 2:404396/81‑1 (MQ=255)
gctgTTTTTCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCa > 1:404436/1‑81 (MQ=255)
tttttCTCCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTAc > 1:404397/1‑81 (MQ=255)
ctcCGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCtt < 1:404398/80‑1 (MQ=255)
cGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCt > 1:404429/1‑81 (MQ=255)
cGTCGCTAAAGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCt < 2:404399/81‑1 (MQ=255)
aaaGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCTGgcagc > 1:404424/1‑80 (MQ=255)
aaGGTAAATAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCTGgcagc < 1:404400/79‑1 (MQ=255)
aaaTAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGCCAATTCTGATGGCGCATTATGCTTTGCTTGCAGCataaaa > 2:404437/1‑80 (MQ=255)
aaTAGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCTGGCAGCAAAAaata < 1:404401/81‑1 (MQ=255)
|
GCAAGCATTCCGCAACCGTCTATTTGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATTGCCCACCTATATTCTTTCCTGCTTTGCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCTGGCAGCAAAAAATA > NC_000913/2371764‑2371919
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
