Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,387,862 |
C→T |
E189K (GAA→AAA) |
yfbN ← |
uncharacterized protein YfbN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,387,862 | 0 | C | T | 88.9%
| 19.7
/ ‑3.5
| 9 | E189K (GAA→AAA) | yfbN | uncharacterized protein YfbN |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (4/4); total (4/5) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 |
AACCTCTCTTGCAAGTTGGTCACAATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTCGGCCTTGTCTTTTAGCTCATCATATCGGATTCTTGCAGTAACCAT > NC_000913/2387785‑2387907
|
aaCCTCTCTTGCAAGTTGGTCACAATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTgg < 2:407066/80‑1 (MQ=255)
aCCTCTCTTGCAAGTTGGTCACAATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGcc < 1:407067/81‑1 (MQ=255)
ttGCAAGTTGGTCACAATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTCGGCCTTGTc < 2:407068/79‑1 (MQ=255)
tGCAAGTTGGTCACAATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGCCTTGTCttt > 1:407084/1‑81 (MQ=255)
acaATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGCCTTGTCTTTTAGCTCATCata > 2:407080/1‑81 (MQ=255)
tGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGCCTTGTCTTTTAGCTCATCATATCg > 2:407081/1‑80 (MQ=255)
taAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGCCTTGTCTTTTAGCTCATCATATCGGATTCTTg > 1:407090/1‑81 (MQ=255)
aTTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGCCTTGTCTTTTAGCTCATCATATCGGATTCTTGCAGTa < 1:407069/81‑1 (MQ=255)
aaTATGTCATCCTCTGTAATCTCGTGAAAATTTTTGGCCTTGTCTTTTAGCTCATCATATCGGATTCTTGCAGTAACCAt < 1:407070/80‑1 (MQ=255)
|
AACCTCTCTTGCAAGTTGGTCACAATGTATTATAAGCATTTTGAATATGTCATCCTCTGTAATCTCGTGAAAATTTTCGGCCTTGTCTTTTAGCTCATCATATCGGATTCTTGCAGTAACCAT > NC_000913/2387785‑2387907
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
