Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,394,103 |
T→A |
Q315L (CAG→CTG) |
nuoL ← |
NADH:quinone oxidoreductase subunit L |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,394,103 | 0 | T | A | 100.0%
| 144.6
/ NA
| 41 | Q315L (CAG→CTG) | nuoL | NADH:quinone oxidoreductase subunit L |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (25/16); total (25/16) |
AAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAG > NC_000913/2394025‑2394176
|
aaGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAgg < 1:408186/81‑1 (MQ=255)
gAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCt > 2:408218/1‑81 (MQ=255)
aaCGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCt > 1:408264/1‑80 (MQ=255)
gcgTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATg > 1:408212/1‑81 (MQ=255)
gTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGt < 1:408187/81‑1 (MQ=255)
atcaAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGt > 2:408222/1‑79 (MQ=255)
atcaAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTa < 1:408188/80‑1 (MQ=255)
aaGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGc < 2:408190/80‑1 (MQ=255)
aaGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGc < 1:408189/80‑1 (MQ=255)
cgcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGAt > 1:408221/1‑81 (MQ=255)
gcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATg > 1:408220/1‑81 (MQ=255)
gcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATg > 1:408223/1‑81 (MQ=255)
gcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATg > 2:408225/1‑81 (MQ=255)
cATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGt < 2:408191/81‑1 (MQ=255)
cATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGt < 1:408192/81‑1 (MQ=255)
aTCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTc < 2:408193/81‑1 (MQ=255)
cccATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCgg > 2:408228/1‑81 (MQ=255)
ccATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCgg < 1:408194/80‑1 (MQ=255)
cATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTc > 1:408219/1‑81 (MQ=255)
gCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTg > 1:408226/1‑80 (MQ=255)
gCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTAc > 1:408236/1‑79 (MQ=255)
cACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAg < 2:408195/81‑1 (MQ=255)
aCGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAgc > 2:408252/1‑81 (MQ=255)
aCGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAgc < 1:408196/81‑1 (MQ=255)
gCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAgcgc > 1:408224/1‑81 (MQ=255)
aaGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCgg > 1:408230/1‑80 (MQ=255)
gAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAAcc > 2:408243/1‑81 (MQ=255)
aGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCg > 1:408239/1‑81 (MQ=255)
ggAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGTGGCAAAACCgg > 2:408238/1‑81 (MQ=255)
gAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc > 1:408233/1‑81 (MQ=255)
aaCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc > 1:408242/1‑80 (MQ=255)
aCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc > 2:408267/1‑79 (MQ=255)
aCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCca < 2:408198/81‑1 (MQ=255)
aCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCca < 2:408197/81‑1 (MQ=255)
aTGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagc < 2:408199/81‑1 (MQ=255)
tAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagc < 2:408200/81‑1 (MQ=255)
aGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcag > 2:408240/1‑79 (MQ=255)
aGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcag > 2:408244/1‑79 (MQ=255)
aGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcag > 2:408247/1‑79 (MQ=255)
gCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagcag > 2:408202/1‑81 (MQ=255)
gCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagcag < 1:408201/81‑1 (MQ=255)
|
AAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAG > NC_000913/2394025‑2394176
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
