Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,395,087 |
G→A |
R87C (CGC→TGC) |
nuoK ← |
NADH:quinone oxidoreductase subunit K |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,395,087 | 0 | G | A | 100.0%
| 142.3
/ NA
| 40 | R87C (CGC→TGC) | nuoK | NADH:quinone oxidoreductase subunit K |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/19); total (21/19) |
TCAATGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGT > NC_000913/2395010‑2395161
|
tcaatGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGcaacg < 1:408443/81‑1 (MQ=255)
atGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAacgacg > 2:408483/1‑81 (MQ=255)
tGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACgg < 1:408444/81‑1 (MQ=255)
tGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACgg > 2:408481/1‑81 (MQ=255)
aaaaTAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTg > 2:408479/1‑79 (MQ=255)
aaaaTAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTg > 1:408487/1‑79 (MQ=255)
aaaaTAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTg > 1:408475/1‑79 (MQ=255)
aataatGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGt < 1:408445/81‑1 (MQ=255)
atGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgc < 1:408446/80‑1 (MQ=255)
aaGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgcagcag > 2:408485/1‑79 (MQ=255)
aaGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgcagcag > 2:408497/1‑79 (MQ=255)
aGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgcagcagca < 2:408447/80‑1 (MQ=255)
aaGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc > 2:408493/1‑80 (MQ=255)
aaGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc > 1:408494/1‑80 (MQ=255)
aGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc > 1:408477/1‑79 (MQ=255)
aGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc > 1:408488/1‑79 (MQ=255)
gCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAg < 1:408448/81‑1 (MQ=255)
aTGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGc > 1:408480/1‑81 (MQ=255)
aTGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGc > 2:408482/1‑81 (MQ=255)
ttCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCg < 2:408449/80‑1 (MQ=255)
cATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGAt < 2:408450/80‑1 (MQ=255)
cATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATa > 2:408495/1‑81 (MQ=255)
ccGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATAccc > 2:408496/1‑79 (MQ=255)
cgcgCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTc < 1:408451/80‑1 (MQ=255)
gcgcATCTCACTTACTGAATCGATGTTCAGGATCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGc < 2:408452/81‑1 (MQ=255)
aTCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCt > 1:408491/1‑81 (MQ=255)
ctcACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgc > 1:408502/1‑81 (MQ=255)
ctcACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgc > 1:408489/1‑81 (MQ=255)
cACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgccg > 2:408503/1‑81 (MQ=255)
aCTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgcc < 2:408453/79‑1 (MQ=255)
cTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCcgcg < 2:408454/80‑1 (MQ=255)
tCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCt > 1:408484/1‑80 (MQ=255)
gATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGAt < 2:408455/81‑1 (MQ=255)
gATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGAt < 2:408456/81‑1 (MQ=255)
aTGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATg < 2:408457/81‑1 (MQ=255)
ttCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGc < 1:408458/80‑1 (MQ=255)
ttCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCg < 1:408459/81‑1 (MQ=255)
gTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAAt > 2:408490/1‑81 (MQ=255)
gTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAAt < 1:408461/81‑1 (MQ=255)
gTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAAt < 1:408460/81‑1 (MQ=255)
cTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGt < 1:408462/80‑1 (MQ=255)
|
TCAATGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGT > NC_000913/2395010‑2395161
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
