Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,395,206 |
A→T |
V47E (GTG→GAG) |
nuoK ← |
NADH:quinone oxidoreductase subunit K |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,395,206 | 0 | A | T | 100.0%
| 132.0
/ NA
| 37 | V47E (GTG→GAG) | nuoK | NADH:quinone oxidoreductase subunit K |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (15/22); total (15/22) |
CCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAG > NC_000913/2395135‑2395282
|
ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGcc < 1:436128/80‑1 (MQ=255)
ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGc < 2:436130/79‑1 (MQ=255)
ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCa < 2:436129/81‑1 (MQ=255)
ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCa < 2:436127/81‑1 (MQ=255)
cgccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAg > 2:436167/1‑81 (MQ=255)
gccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAgc < 2:436132/81‑1 (MQ=255)
gccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAgc < 2:436131/81‑1 (MQ=255)
cgcgAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAgcgc > 1:436179/1‑81 (MQ=255)
gcgAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGcgcg < 1:436133/81‑1 (MQ=255)
gAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGcgcg > 1:436182/1‑79 (MQ=255)
gAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGcgcg > 1:436186/1‑79 (MQ=255)
ggCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAgg < 1:436134/81‑1 (MQ=255)
gCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGc < 2:436135/81‑1 (MQ=255)
aTGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGtt > 2:436170/1‑80 (MQ=255)
gCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAAt > 1:436172/1‑80 (MQ=255)
gCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATc < 2:436136/81‑1 (MQ=255)
cGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCa < 1:436137/81‑1 (MQ=255)
tGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGAttt < 2:436138/81‑1 (MQ=255)
tACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTc < 1:436139/80‑1 (MQ=255)
tCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAc < 2:436140/81‑1 (MQ=255)
cACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAc > 1:436183/1‑80 (MQ=255)
aCCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAcc > 1:436190/1‑80 (MQ=255)
aCCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAcc < 2:436141/80‑1 (MQ=255)
ccGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAAc < 2:436142/81‑1 (MQ=255)
gTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACa > 1:436181/1‑80 (MQ=255)
ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaaa > 1:436184/1‑81 (MQ=255)
ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaa < 1:436144/80‑1 (MQ=255)
ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaa < 2:436143/80‑1 (MQ=255)
gCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAAcagca < 2:436145/80‑1 (MQ=255)
gTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCg < 1:436146/81‑1 (MQ=255)
cTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACg > 1:436178/1‑80 (MQ=255)
ttCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGa > 2:436185/1‑81 (MQ=255)
tCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGa > 2:436192/1‑80 (MQ=255)
cGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAc > 1:436199/1‑79 (MQ=255)
cGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAc > 2:436189/1‑79 (MQ=255)
cGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCa < 2:436147/81‑1 (MQ=255)
gACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAg < 1:436148/81‑1 (MQ=255)
|
CCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAG > NC_000913/2395135‑2395282
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
