Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,399,514 |
T→C |
K218E (AAA→GAA) |
nuoG ← |
NADH:quinone oxidoreductase subunit G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,399,514 | 0 | T | C | 100.0%
| 147.2
/ NA
| 40 | K218E (AAA→GAA) | nuoG | NADH:quinone oxidoreductase subunit G |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/23); total (17/23) |
GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATT > NC_000913/2399437‑2399591
|
gTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACg < 1:437140/81‑1 (MQ=255)
aCGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGtt > 2:437190/1‑81 (MQ=255)
aCGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGtt > 1:437191/1‑81 (MQ=255)
gTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTg > 2:437199/1‑80 (MQ=255)
tCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa > 2:437201/1‑81 (MQ=255)
tCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa > 1:437213/1‑81 (MQ=255)
cACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa < 2:437141/80‑1 (MQ=255)
aCCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACg < 1:437143/81‑1 (MQ=255)
aCCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACg < 2:437142/81‑1 (MQ=255)
ggggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTc > 2:437198/1‑81 (MQ=255)
ggggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTc > 1:437204/1‑81 (MQ=255)
ggggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTc < 2:437144/81‑1 (MQ=255)
ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCgg < 1:437145/81‑1 (MQ=255)
cTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAg > 2:437194/1‑81 (MQ=255)
aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGt < 2:437146/79‑1 (MQ=255)
tGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCg < 1:437148/81‑1 (MQ=255)
tGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCg < 2:437147/81‑1 (MQ=255)
cAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTg > 2:437207/1‑81 (MQ=255)
gCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTc < 1:437149/81‑1 (MQ=255)
gATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGt < 1:437150/81‑1 (MQ=255)
aTGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTa < 2:437151/81‑1 (MQ=255)
aaCATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATa < 1:437152/81‑1 (MQ=255)
aCATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATAc < 2:437153/81‑1 (MQ=255)
tGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCg > 1:437210/1‑81 (MQ=255)
cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCgg < 2:437155/80‑1 (MQ=255)
cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGt < 1:437154/81‑1 (MQ=255)
aGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGc < 1:437156/81‑1 (MQ=255)
cTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAAtt < 1:437157/81‑1 (MQ=255)
ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc < 1:437158/80‑1 (MQ=255)
ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc > 1:437200/1‑80 (MQ=255)
aaaCTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAg > 1:437208/1‑81 (MQ=255)
gCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAc > 2:437203/1‑81 (MQ=255)
cATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAcc > 2:437220/1‑81 (MQ=255)
tatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCgg < 2:437160/81‑1 (MQ=255)
tatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCgg < 1:437159/81‑1 (MQ=255)
tCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGa > 2:437209/1‑80 (MQ=255)
cccATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGaa > 1:437205/1‑80 (MQ=255)
cccATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGaa > 2:437222/1‑80 (MQ=255)
ccATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGaaaa < 1:437161/81‑1 (MQ=255)
aTTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAAtt < 1:437162/81‑1 (MQ=255)
|
GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATT > NC_000913/2399437‑2399591
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
