Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,399,514 |
T→C |
K218E (AAA→GAA) |
nuoG ← |
NADH:quinone oxidoreductase subunit G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,399,514 | 0 | T | C | 100.0%
| 130.7
/ NA
| 37 | K218E (AAA→GAA) | nuoG | NADH:quinone oxidoreductase subunit G |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/21); total (16/21) |
GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGG > NC_000913/2399437‑2399585
|
gTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACg > 1:409490/1‑81 (MQ=255)
tAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACgg > 2:409488/1‑81 (MQ=255)
tCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa > 2:409479/1‑81 (MQ=255)
cGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCt > 2:409470/1‑81 (MQ=255)
cGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCt < 1:409427/81‑1 (MQ=255)
gggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCg > 2:409462/1‑81 (MQ=255)
ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCgg < 2:409428/81‑1 (MQ=255)
ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCg > 2:409481/1‑80 (MQ=255)
gATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTg > 2:409473/1‑81 (MQ=255)
aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTg < 2:409430/80‑1 (MQ=255)
aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGc < 1:409429/81‑1 (MQ=255)
aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGc > 2:409471/1‑81 (MQ=255)
tGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCg < 1:409431/81‑1 (MQ=255)
gTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGt > 1:409493/1‑81 (MQ=255)
ttACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGtt > 2:409466/1‑81 (MQ=255)
gCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATAGCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTc < 2:409432/81‑1 (MQ=255)
ccGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCg > 1:409475/1‑81 (MQ=255)
ggAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTaaa > 1:409477/1‑81 (MQ=255)
ggAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGCTGTAACGCTCGGAGTGCGTTTTGTCGGTaaa > 2:409478/1‑81 (MQ=255)
gAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTaaa < 2:409434/80‑1 (MQ=255)
gAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAAt < 1:409433/81‑1 (MQ=255)
cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGt < 2:409435/81‑1 (MQ=255)
gCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTc < 1:409438/79‑1 (MQ=255)
gCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCg < 1:409436/80‑1 (MQ=255)
gCAGATGCTCGGCACAAACTGCGTATCCCATTCACAGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTc < 2:409437/79‑1 (MQ=255)
aGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGc < 1:409439/81‑1 (MQ=255)
cTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAAtt > 1:409485/1‑81 (MQ=255)
cGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc > 2:409486/1‑81 (MQ=255)
ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc > 2:409498/1‑80 (MQ=255)
ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc < 2:409440/80‑1 (MQ=255)
gcgcAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGa < 2:409441/81‑1 (MQ=255)
gcAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGAcc < 1:409442/81‑1 (MQ=255)
cTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGtt < 2:409443/81‑1 (MQ=255)
cATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAc < 1:409444/80‑1 (MQ=255)
cATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAc < 1:409445/80‑1 (MQ=255)
atatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCg < 2:409446/81‑1 (MQ=255)
tatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCgg < 1:409447/81‑1 (MQ=255)
|
GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGG > NC_000913/2399437‑2399585
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
