Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,395,206 |
A→T |
V47E (GTG→GAG) |
nuoK ← |
NADH:quinone oxidoreductase subunit K |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,395,206 | 0 | A | T | 100.0%
| 64.2
/ NA
| 19 | V47E (GTG→GAG) | nuoK | NADH:quinone oxidoreductase subunit K |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/8); total (11/8) |
TCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAC > NC_000913/2395131‑2395279
|
tCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAg > 2:408501/1‑81 (MQ=255)
cTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAgg > 2:408511/1‑81 (MQ=255)
cTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAg < 2:408472/80‑1 (MQ=255)
gAGGCTGATGGCGAGAATGTACATCACCTGACC‑TCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCAAAGGCCAGCGCGGAg > 1:408500/1‑81 (MQ=255)
gATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGt > 1:408506/1‑80 (MQ=255)
aTGGCGAGAATGTACATCACCTGACCGTCGATCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGtt < 2:408473/80‑1 (MQ=255)
agaATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCAt > 1:408517/1‑80 (MQ=255)
agaATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCAt > 1:408519/1‑80 (MQ=255)
gaATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGa < 1:408474/81‑1 (MQ=255)
aTCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGa < 2:408475/81‑1 (MQ=255)
aCCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAcc < 1:408476/80‑1 (MQ=255)
cTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAAt < 2:408477/81‑1 (MQ=255)
gACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATc > 1:408513/1‑80 (MQ=255)
gTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACa > 2:408512/1‑80 (MQ=255)
ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaaa > 2:408516/1‑81 (MQ=255)
ggCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAAcag > 2:408521/1‑79 (MQ=255)
gCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAAcag < 2:408478/78‑1 (MQ=255)
cGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATa < 1:408479/80‑1 (MQ=255)
gACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAc > 1:408514/1‑78 (MQ=255)
|
TCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAC > NC_000913/2395131‑2395279
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
