Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,430,546 |
G→A |
T73I (ACC→ATC) |
cvpA ← |
colicin V production protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,430,546 | 0 | G | A | 100.0%
| 114.9
/ NA
| 33 | T73I (ACC→ATC) | cvpA | colicin V production protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/19); total (14/19) |
CCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGG > NC_000913/2430469‑2430623
|
cccGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAg < 1:414979/81‑1 (MQ=255)
ccGATCGGTGCCTGACAACCCCGTTTTCTCCGCCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGc < 2:414980/81‑1 (MQ=255)
cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCg < 2:414981/81‑1 (MQ=255)
tCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATa < 1:414982/81‑1 (MQ=255)
ggTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATaaa > 2:415030/1‑81 (MQ=255)
aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTAc < 2:414983/79‑1 (MQ=255)
aaCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACcgc > 2:415036/1‑80 (MQ=255)
ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGAt < 1:414984/81‑1 (MQ=255)
cGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGc < 1:414985/81‑1 (MQ=255)
tctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATcc > 1:415040/1‑81 (MQ=255)
ccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTc < 1:414986/81‑1 (MQ=255)
caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTc > 1:415041/1‑80 (MQ=255)
caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg < 1:414987/81‑1 (MQ=255)
caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg < 2:414988/81‑1 (MQ=255)
aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg > 2:415035/1‑80 (MQ=255)
aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg > 1:415034/1‑80 (MQ=255)
ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAAc > 2:415039/1‑79 (MQ=255)
tATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTc > 1:415038/1‑81 (MQ=255)
cACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTc > 1:415062/1‑81 (MQ=255)
aCGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTc < 2:414989/80‑1 (MQ=255)
cGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCtt > 1:415037/1‑81 (MQ=255)
gAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCtt > 2:415046/1‑80 (MQ=255)
gAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTc < 2:414990/81‑1 (MQ=255)
cACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGcc > 2:415032/1‑81 (MQ=255)
cGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCg < 2:414991/81‑1 (MQ=255)
gATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGt < 1:414992/81‑1 (MQ=255)
aTAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTa < 1:414994/81‑1 (MQ=255)
aTAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTa < 1:414993/81‑1 (MQ=255)
aCCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCa < 2:414995/81‑1 (MQ=255)
aCGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACt < 2:414996/81‑1 (MQ=255)
cAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAg > 1:415042/1‑81 (MQ=255)
cAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAg > 2:415053/1‑81 (MQ=255)
aGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAgg < 1:414997/81‑1 (MQ=255)
|
CCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGG > NC_000913/2430469‑2430623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
