Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,565,882 |
G→A |
P335S (CCG→TCG) |
eutA ← |
reactivating factor for ethanolamine ammonia‑lyase EutBC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,565,882 | 0 | G | A | 100.0%
| 108.3
/ NA
| 31 | P335S (CCG→TCG) | eutA | reactivating factor for ethanolamine ammonia‑lyase EutBC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/18); total (13/18) |
CTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGGCGC > NC_000913/2565805‑2565959
|
cTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAg > 1:437538/1‑81 (MQ=255)
aaTCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTg < 1:437501/81‑1 (MQ=255)
aaTCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTg > 2:437541/1‑81 (MQ=255)
cAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTAc > 1:437550/1‑81 (MQ=255)
aGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACg < 2:437502/81‑1 (MQ=255)
cgcCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGcc < 2:437503/81‑1 (MQ=255)
gTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTc > 2:437549/1‑79 (MQ=255)
tGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGc > 2:437542/1‑81 (MQ=255)
aGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGc > 1:437545/1‑77 (MQ=255)
ggCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAt < 1:437504/81‑1 (MQ=255)
ggCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAt > 2:437546/1‑81 (MQ=255)
ggCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAt < 1:437505/81‑1 (MQ=255)
gCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAtt < 1:437506/81‑1 (MQ=255)
ccccACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATtgt < 1:437507/81‑1 (MQ=255)
ccaccaGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgc > 2:437543/1‑81 (MQ=255)
ccaccaGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgc < 1:437508/81‑1 (MQ=255)
accaGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgctg < 1:437509/81‑1 (MQ=255)
gATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgctgc > 1:437548/1‑78 (MQ=255)
atcaATCGGGATCGCCACCGGCAAATTGCGCGGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAg < 2:437511/81‑1 (MQ=255)
atcaATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAg < 2:437510/81‑1 (MQ=255)
aaTCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGt < 1:437512/81‑1 (MQ=255)
aTCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTa < 1:437513/81‑1 (MQ=255)
ggATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATgcg < 2:437514/81‑1 (MQ=255)
gCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGcgcgccc < 2:437515/81‑1 (MQ=255)
cGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGcgcgcccgcgcc < 2:437516/81‑1 (MQ=255)
ggCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGcgcgcccgcgcc > 2:437547/1‑80 (MQ=255)
cgcAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGt < 1:437517/80‑1 (MQ=255)
gcAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg < 2:437518/81‑1 (MQ=255)
gcAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg > 2:437558/1‑81 (MQ=255)
aGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGgcg > 2:437556/1‑81 (MQ=255)
gCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGgcgc > 1:437551/1‑81 (MQ=255)
|
CTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGGCGC > NC_000913/2565805‑2565959
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
