Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,578,163 |
T→C |
N72S (AAC→AGC) |
maeB ← |
malate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,578,163 | 0 | T | C | 100.0%
| 79.6
/ NA
| 23 | N72S (AAC→AGC) | maeB | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/12); total (11/12) |
ACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGA > NC_000913/2578091‑2578237
|
aCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATc < 1:440207/81‑1 (MQ=255)
gCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCAc > 1:440254/1‑81 (MQ=255)
tGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCTCTAGAGATCACCGcca > 2:440226/1‑81 (MQ=255)
cTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATcaccaccac > 2:440245/1‑79 (MQ=255)
cTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGccacca < 2:440208/81‑1 (MQ=255)
cATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGtt < 1:440209/81‑1 (MQ=255)
tgcctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCg < 2:440210/80‑1 (MQ=255)
cctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGt < 1:440211/80‑1 (MQ=255)
ctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat < 1:440212/81‑1 (MQ=255)
ctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat < 1:440213/81‑1 (MQ=255)
gccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat > 2:440238/1‑81 (MQ=255)
gcCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGc > 1:440244/1‑81 (MQ=255)
gTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa > 1:440241/1‑81 (MQ=255)
ccTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACgg < 1:440214/80‑1 (MQ=255)
aCCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtt > 1:440239/1‑81 (MQ=255)
cccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtttt > 1:440243/1‑81 (MQ=255)
ccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt < 1:440217/81‑1 (MQ=255)
ccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt < 1:440216/81‑1 (MQ=255)
ccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt < 1:440215/81‑1 (MQ=255)
aGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCg < 2:440218/81‑1 (MQ=255)
ccgccgTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt > 1:440249/1‑81 (MQ=255)
gccgTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATGTc > 2:440251/1‑80 (MQ=255)
gTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGa > 1:440235/1‑81 (MQ=255)
|
ACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGA > NC_000913/2578091‑2578237
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
