Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,646,247 |
G→A |
P360L (CCG→CTG) |
pbpC ← |
peptidoglycan glycosyltransferase PbpC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,646,247 | 0 | G | A | 100.0%
| 159.3
/ NA
| 45 | P360L (CCG→CTG) | pbpC | peptidoglycan glycosyltransferase PbpC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/22); total (23/22) |
GGGCCATGAAAACCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCGGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCGATCGGATCGAATT > NC_000913/2646169‑2646323
|
gggCCATGAAAACCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCagg > 2:453301/1‑81 (MQ=255)
gggCCATGAAAACCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCagg > 2:453298/1‑81 (MQ=255)
gggCCATGAAAACCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCagg > 1:453317/1‑81 (MQ=255)
gCCATGAAAACCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTg < 1:453256/81‑1 (MQ=255)
aaCCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGc > 1:453302/1‑81 (MQ=255)
aCCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGcc < 1:453257/81‑1 (MQ=255)
gCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTc < 1:453258/81‑1 (MQ=255)
gCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTc < 1:453259/81‑1 (MQ=255)
tCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATc > 1:453307/1‑80 (MQ=255)
aaaGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAg < 2:453260/81‑1 (MQ=255)
ccTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGa > 1:453315/1‑81 (MQ=255)
cTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCACCAGAc < 1:453261/81‑1 (MQ=255)
gTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCa > 1:453329/1‑80 (MQ=255)
tCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCAt > 2:453310/1‑80 (MQ=255)
cGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATa > 2:453312/1‑80 (MQ=255)
gATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATaaa > 2:453306/1‑81 (MQ=255)
gATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATaa < 2:453262/80‑1 (MQ=255)
tAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATaaac > 2:453311/1‑80 (MQ=255)
aCCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACgg > 2:453289/1‑81 (MQ=255)
aCCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACgg > 1:453336/1‑81 (MQ=255)
aCCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACgg > 1:453339/1‑81 (MQ=255)
ccGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGt < 1:453263/81‑1 (MQ=255)
tGCGCCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGa < 1:453264/81‑1 (MQ=255)
gcgcCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAg > 2:453320/1‑81 (MQ=255)
cgcCGGGGGACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAg > 1:453297/1‑80 (MQ=255)
cGGGGGACGTCTTGCAGCAGTGATGCCAGGTAGATCAAGCCTTCATCCAGCACCAGACCATAAACAAACGGTTTGAGCACt < 2:453265/81‑1 (MQ=255)
gggggACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACt > 2:453316/1‑80 (MQ=255)
ggggACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGa > 2:453327/1‑81 (MQ=255)
gggACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGAt < 1:453268/81‑1 (MQ=255)
gggACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGAt < 2:453267/81‑1 (MQ=255)
gggACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCACCAGACCATAAACAAACGGTTTGAGCACTGAt < 1:453266/81‑1 (MQ=255)
ggACGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATc > 1:453325/1‑81 (MQ=255)
cGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTg < 1:453272/81‑1 (MQ=255)
cGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTg < 1:453270/81‑1 (MQ=255)
cGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTg < 1:453269/81‑1 (MQ=255)
cGTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTg < 2:453271/81‑1 (MQ=255)
gTCTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTgg > 1:453324/1‑81 (MQ=255)
cTTGCAGCAGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGc > 2:453308/1‑80 (MQ=255)
ttGCAGCAGTGATGCCAGGTGGATCAATCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCga > 2:453321/1‑81 (MQ=255)
gcagcaGTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCg < 2:453273/78‑1 (MQ=255)
cagcagTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCgatc < 2:453275/80‑1 (MQ=255)
cagcagTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCgatc < 1:453274/80‑1 (MQ=255)
agcagTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCgatcg > 1:453319/1‑80 (MQ=255)
gcagTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCgatcgga > 1:453309/1‑81 (MQ=255)
cagTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCgatcggat > 1:453318/1‑81 (MQ=255)
agTGATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCgatcggatc < 1:453276/81‑1 (MQ=255)
gATGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCGATCGGATCGaa > 2:453313/1‑81 (MQ=255)
tGCCAGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCGATCGGATCGAAtt < 2:453277/81‑1 (MQ=255)
|
GGGCCATGAAAACCGCTATCAAAGTTACCTGGTCGATAATCACCGGTGCGCCGGGGGACGTCTTGCAGCAGTGATGCCGGGTGGATCAAGCCTTCATCCAGCGCCAGACCATAAACAAACGGTTTGAGCACTGATCCTGGCGATCGGATCGAATT > NC_000913/2646169‑2646323
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
