Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,647,072 |
T→C |
D85G (GAT→GGT) |
pbpC ← |
peptidoglycan glycosyltransferase PbpC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,647,072 | 0 | T | C | 100.0%
| 116.3
/ NA
| 33 | D85G (GAT→GGT) | pbpC | peptidoglycan glycosyltransferase PbpC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/17); total (16/17) |
CCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGT > NC_000913/2646998‑2647147
|
ccGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCa > 2:484925/1‑80 (MQ=255)
ccGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCa < 2:484887/80‑1 (MQ=255)
cGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATa < 1:484888/81‑1 (MQ=255)
gAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATaa < 1:484889/81‑1 (MQ=255)
gagaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGAt > 1:484922/1‑81 (MQ=255)
gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAgcg < 2:484890/78‑1 (MQ=255)
gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAg < 1:484891/76‑1 (MQ=255)
aTGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTc > 1:484937/1‑79 (MQ=255)
ctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGt < 1:484892/81‑1 (MQ=255)
cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAAc < 1:484893/81‑1 (MQ=255)
cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAAc < 1:484895/81‑1 (MQ=255)
cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAAc < 2:484894/81‑1 (MQ=255)
cgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTgg < 2:484896/81‑1 (MQ=255)
cgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTg > 2:484934/1‑80 (MQ=255)
gagaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACa > 1:484924/1‑81 (MQ=255)
agaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACAt > 1:484943/1‑81 (MQ=255)
tGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTc < 1:484897/81‑1 (MQ=255)
cACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTg < 1:484900/80‑1 (MQ=255)
cACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTg < 2:484899/80‑1 (MQ=255)
cACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGt > 2:484929/1‑81 (MQ=255)
ccccccGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGt > 2:484928/2‑80 (MQ=255)
ccccGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTAc > 2:800540/1‑81 (MQ=255)
cGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCgg < 1:484901/81‑1 (MQ=255)
ggATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa > 1:484944/1‑81 (MQ=255)
gATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa > 2:484926/1‑80 (MQ=255)
gATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa > 2:484933/1‑80 (MQ=255)
gATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa > 1:484949/1‑80 (MQ=255)
aTGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGAt > 1:484927/1‑80 (MQ=255)
ccAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGcc < 1:484902/80‑1 (MQ=255)
cAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGc > 2:484942/1‑78 (MQ=255)
gAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGAt > 1:484938/1‑81 (MQ=255)
ccAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGcc < 2:484903/81‑1 (MQ=255)
aGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGt < 1:484904/81‑1 (MQ=255)
|
CCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGT > NC_000913/2646998‑2647147
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
