Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,667,155 |
G→A |
A231V (GCA→GTA) |
hcaT ← |
putative 3‑phenylpropionate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,667,155 | 0 | G | A | 100.0%
| 87.8
/ NA
| 26 | A231V (GCA→GTA) | hcaT | putative 3‑phenylpropionate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/16); total (10/16) |
CTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAG > NC_000913/2667077‑2667229
|
cTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctacc > 2:457370/1‑81 (MQ=255)
aGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctacctgc > 1:457364/1‑81 (MQ=255)
aaGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTa > 2:457362/1‑80 (MQ=255)
aGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTaaa < 1:457325/81‑1 (MQ=255)
gATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAAt > 1:457367/1‑81 (MQ=255)
aaTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGgc > 2:457351/1‑81 (MQ=255)
tGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGgcgc < 1:457326/81‑1 (MQ=255)
aCTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTa < 1:457327/81‑1 (MQ=255)
ccGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAAc > 1:457363/1‑81 (MQ=255)
cGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAAc < 2:457328/80‑1 (MQ=255)
gCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCg > 1:457361/1‑81 (MQ=255)
ccaccaCGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGt < 1:457329/81‑1 (MQ=255)
acGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAg > 2:457365/1‑81 (MQ=255)
ccAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCg > 2:457366/1‑81 (MQ=255)
aGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCa < 2:457330/81‑1 (MQ=255)
gCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt < 2:457331/81‑1 (MQ=255)
aaTACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt < 2:457332/81‑1 (MQ=255)
aaTACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt < 2:457333/81‑1 (MQ=255)
tACCCCACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGc < 2:457334/81‑1 (MQ=255)
ccccACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCa < 2:457335/80‑1 (MQ=255)
cACCGCCGAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa < 2:457336/81‑1 (MQ=255)
ccgccgAGGCCGAGTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaac < 1:457337/81‑1 (MQ=255)
gccgaggccgagTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaaca > 2:457380/1‑80 (MQ=255)
ccgaggccgagTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa > 1:457372/1‑81 (MQ=255)
ggccgagTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa < 1:457338/81‑1 (MQ=255)
gagTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc > 2:457369/1‑80 (MQ=255)
gTAGCCAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg < 2:457339/81‑1 (MQ=255)
ccAGCTACCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAg < 2:457340/81‑1 (MQ=255)
|
CTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAG > NC_000913/2667077‑2667229
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
