Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,677,674 |
C→A |
D230Y (GAT→TAT) |
yphE ← |
putative ABC transporter ATP‑binding protein YphE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,677,674 | 0 | C | A | 100.0%
| 102.2
/ NA
| 29 | D230Y (GAT→TAT) | yphE | putative ABC transporter ATP‑binding protein YphE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/13); total (16/13) |
CGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC > NC_000913/2677597‑2677745
|
cGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagcc > 2:491353/1‑81 (MQ=255)
ggCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagccc > 2:491365/1‑81 (MQ=255)
ggCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagccc < 2:491333/81‑1 (MQ=255)
gcgcAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagcccg > 2:491381/1‑81 (MQ=255)
tatCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCt < 1:491335/81‑1 (MQ=255)
tCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTg < 1:491337/80‑1 (MQ=255)
tCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGa < 1:491336/81‑1 (MQ=255)
gTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATc > 1:491379/1‑81 (MQ=255)
gTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATc < 2:491338/81‑1 (MQ=255)
tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCgcgc < 1:491339/81‑1 (MQ=255)
gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCa > 1:491399/1‑80 (MQ=255)
gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt < 1:491340/81‑1 (MQ=255)
cgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt > 1:491394/1‑80 (MQ=255)
gcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATaa > 2:491389/1‑81 (MQ=255)
gcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATaa < 2:491341/81‑1 (MQ=255)
ccGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACg > 1:491392/1‑81 (MQ=255)
cGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACgg > 2:491393/1‑81 (MQ=255)
gCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTgg > 1:491388/1‑81 (MQ=255)
cATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGc < 1:491342/81‑1 (MQ=255)
aTCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGca > 1:491382/1‑81 (MQ=255)
cacaATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACaggagg > 2:491376/1‑80 (MQ=255)
atgatgCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATgcg > 2:491390/1‑81 (MQ=255)
gCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGaa > 2:491374/1‑81 (MQ=255)
cGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAAt < 1:491343/81‑1 (MQ=255)
gTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAAtt > 2:491386/1‑81 (MQ=255)
gAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttct < 1:491344/81‑1 (MQ=255)
ggTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttcttc < 2:491345/81‑1 (MQ=255)
ttttCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATc > 2:491385/1‑81 (MQ=255)
cGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCgg < 1:491346/80‑1 (MQ=255)
cGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGt > 1:491403/1‑81 (MQ=255)
gAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTg > 1:491406/1‑81 (MQ=255)
aGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTgg > 1:491373/1‑81 (MQ=255)
gCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGc < 2:491347/81‑1 (MQ=255)
|
CGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC > NC_000913/2677597‑2677745
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
