Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,677,674 |
C→A |
D230Y (GAT→TAT) |
yphE ← |
putative ABC transporter ATP‑binding protein YphE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,677,674 | 0 | C | A | 100.0%
| 105.7
/ NA
| 31 | D230Y (GAT→TAT) | yphE | putative ABC transporter ATP‑binding protein YphE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (15/16); total (15/16) |
CCGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC > NC_000913/2677596‑2677745
|
ccGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagc < 1:459474/81‑1 (MQ=255)
ggCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagccc > 1:459516/1‑81 (MQ=255)
gcgcAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagcccg > 2:459536/1‑81 (MQ=255)
cAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCa > 2:459475/1‑81 (MQ=255)
atatCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCAcc < 1:459476/81‑1 (MQ=255)
cAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGAc > 1:459524/1‑81 (MQ=255)
cGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCAt < 2:459477/81‑1 (MQ=255)
gATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATcgc > 2:459523/1‑81 (MQ=255)
aTCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATcgc < 1:459478/80‑1 (MQ=255)
tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCgcgc < 2:459479/81‑1 (MQ=255)
tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCgcgc < 1:459480/81‑1 (MQ=255)
tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTCCGAGCATCACATAGCCCGCCACCTGACCATCgcgc < 1:459481/81‑1 (MQ=255)
gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCa > 2:459515/1‑80 (MQ=255)
gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt < 1:459482/81‑1 (MQ=255)
cgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt > 2:459522/1‑80 (MQ=255)
aGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTg > 2:459521/1‑81 (MQ=255)
gCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTgg < 2:459483/81‑1 (MQ=255)
tCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGcac < 2:459484/81‑1 (MQ=255)
gCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACag > 1:459528/1‑80 (MQ=255)
gCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACag > 1:459517/1‑80 (MQ=255)
aaTATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAAt < 2:459485/81‑1 (MQ=255)
tgatgCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGACACAGGAGGCAATgcg < 2:459486/80‑1 (MQ=255)
gatgCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATgcggc > 1:459541/1‑81 (MQ=255)
gAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttct > 1:459557/1‑81 (MQ=255)
gAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttc < 2:459487/80‑1 (MQ=255)
aGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCCTAACGGTGGCACAGGAGGCAATGCGGCGAATttctt < 1:459488/81‑1 (MQ=255)
ggTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttcttc > 2:459532/1‑81 (MQ=255)
gtgtTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTcc < 1:459489/81‑1 (MQ=255)
gtTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCAt > 1:459533/1‑81 (MQ=255)
tttCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATcc > 1:459530/1‑81 (MQ=255)
ttCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCg > 2:459535/1‑81 (MQ=255)
gAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTg < 2:459490/81‑1 (MQ=255)
aGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTgg < 2:459491/81‑1 (MQ=255)
gCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGc > 1:459525/1‑81 (MQ=255)
|
CCGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC > NC_000913/2677596‑2677745
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
