Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,687,164 |
T→A |
Q82L (CAA→CTA) |
glnB ← |
nitrogen regulatory protein PII‑1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,687,164 | 0 | T | A | 100.0%
| 104.6
/ NA
| 30 | Q82L (CAA→CTA) | glnB | nitrogen regulatory protein PII‑1 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (15/15); total (15/15) |
TCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCG > NC_000913/2687092‑2687239
|
tCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTg > 2:461240/1‑81 (MQ=255)
cACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCg < 2:461213/78‑1 (MQ=255)
cACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGt < 1:461214/79‑1 (MQ=255)
aCCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGc > 1:461250/1‑80 (MQ=255)
ggTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGCCACCGATTTTGCCGGTTAGCGCCGTGCGaa > 2:461245/1‑80 (MQ=255)
gTACGGATGCGAACGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGaat > 1:461228/1‑80 (MQ=255)
gATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATgg > 2:461244/1‑80 (MQ=255)
tGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGaca > 1:461257/1‑80 (MQ=255)
cccGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAgg < 2:461215/81‑1 (MQ=255)
ccACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCg < 1:461216/80‑1 (MQ=255)
ccACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCg < 2:461218/80‑1 (MQ=255)
ccACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCg < 1:461217/80‑1 (MQ=255)
gTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAAt < 2:486508/81‑1 (MQ=255)
cAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgt > 1:461263/1‑81 (MQ=255)
gACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgtcgtc > 2:461247/1‑81 (MQ=255)
gACGAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgtcgtc > 2:461253/1‑81 (MQ=255)
gAAGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCgg > 1:461264/1‑81 (MQ=255)
aGATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTa > 2:461255/1‑81 (MQ=255)
gATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTAc < 2:461219/81‑1 (MQ=255)
gATTTTACCGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTAc < 2:461220/81‑1 (MQ=255)
cGTCACCGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtct < 2:461221/81‑1 (MQ=255)
cGATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAttt < 1:461222/81‑1 (MQ=255)
gATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtttt > 1:461249/1‑81 (MQ=255)
gATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtttt < 1:461223/81‑1 (MQ=255)
gATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtttt > 2:461265/1‑81 (MQ=255)
gATTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtttt > 1:461256/1‑81 (MQ=255)
aTTTTGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtttt > 1:461293/1‑80 (MQ=255)
tGCCGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACt < 1:461224/80‑1 (MQ=255)
cGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCg < 1:461225/81‑1 (MQ=255)
cGGTTAGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCg < 2:461226/81‑1 (MQ=255)
|
TCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCG > NC_000913/2687092‑2687239
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
