Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,695,642 |
A→G |
intergenic (‑99/‑159) |
purL ← / → mltF |
phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,695,642 | 0 | A | G | 100.0%
| 49.3
/ NA
| 15 | intergenic (‑99/‑159) | purL/mltF | phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/9); total (6/9) |
CGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTAC > NC_000913/2695567‑2695713
|
cGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGgaaatgaaat > 2:495140/1‑80 (MQ=255)
cGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGgaaatgaaat > 2:495131/1‑80 (MQ=255)
cGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGgaaatgaaat > 1:495133/1‑80 (MQ=255)
ggTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATc < 1:495107/80‑1 (MQ=255)
cccAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATc < 2:495108/80‑1 (MQ=255)
aaGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTg > 1:495130/1‑80 (MQ=255)
gggggggAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAAt < 2:495109/81‑1 (MQ=255)
aaCGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAAcg < 1:495110/81‑1 (MQ=255)
cGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAAcgcg > 1:495146/1‑81 (MQ=255)
aTTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTgccgcc > 2:495138/1‑80 (MQ=255)
aTTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCa < 1:495111/81‑1 (MQ=255)
gATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAg > 1:495141/1‑81 (MQ=255)
aTGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaataa < 1:495112/79‑1 (MQ=255)
cGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTg < 2:495113/81‑1 (MQ=255)
gAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTgg > 1:495142/1‑81 (MQ=255)
ccGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCa < 2:495114/81‑1 (MQ=255)
ccGTGGAAATGAAATCACCATCGTGAATTAGCAACTCGTTCCGCCTATTTCTGTAATAAGTTTCCATCTGTCTCATGTTTa > 2:495143/2‑81 (MQ=255)
cGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTAc < 1:495115/81‑1 (MQ=255)
|
CGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTAC > NC_000913/2695567‑2695713
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
