Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,695,642 |
A→G |
intergenic (‑99/‑159) |
purL ← / → mltF |
phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,695,642 | 0 | A | G | 100.0%
| 56.0
/ NA
| 17 | intergenic (‑99/‑159) | purL/mltF | phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/11); total (6/11) |
TACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAA > NC_000913/2695572‑2695719
|
tACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCAt < 2:462961/81‑1 (MQ=255)
tACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCAt < 1:462962/81‑1 (MQ=255)
ccAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGt < 1:462963/81‑1 (MQ=255)
gAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCaa > 1:462983/1‑81 (MQ=255)
cGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTgcc > 2:462987/1‑81 (MQ=255)
aTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaa < 2:462964/81‑1 (MQ=255)
tGATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaat > 1:462977/1‑79 (MQ=255)
gATGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAg > 2:462990/1‑81 (MQ=255)
aTGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaata < 2:462965/78‑1 (MQ=255)
aTGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaat < 1:462966/77‑1 (MQ=255)
tGCGCTGACGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGtt < 2:462967/81‑1 (MQ=255)
aCGAAACCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATc < 2:462968/80‑1 (MQ=255)
aCCGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcgc < 2:462969/81‑1 (MQ=255)
cGTTTGCGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAg > 2:462984/1‑81 (MQ=255)
cGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTa < 2:462971/80‑1 (MQ=255)
cGTGGAAATGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTa < 1:462970/80‑1 (MQ=255)
aaTGAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCaaaa > 2:462992/1‑81 (MQ=255)
|
TACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAA > NC_000913/2695572‑2695719
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
