Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,707,956 |
G→T |
R267S (CGC→AGC) |
rseB ← |
anti‑sigma factor stabilizing protein RseB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,707,956 | 0 | G | T | 100.0%
| 146.0
/ NA
| 41 | R267S (CGC→AGC) | rseB | anti‑sigma factor stabilizing protein RseB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/22); total (19/22) |
GGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGT > NC_000913/2707879‑2708028
|
ggCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGt > 1:465291/1‑80 (MQ=255)
gTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAAc > 2:465307/1‑81 (MQ=255)
gTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAAc < 1:465250/81‑1 (MQ=255)
aTCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGt < 1:465251/80‑1 (MQ=255)
tCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGttt < 2:465253/81‑1 (MQ=255)
tCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGttt < 2:465254/81‑1 (MQ=255)
tCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGtt < 1:465252/80‑1 (MQ=255)
cACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTa > 1:465300/1‑81 (MQ=255)
aCGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTAc > 2:465311/1‑81 (MQ=255)
gCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGaa < 2:465255/81‑1 (MQ=255)
cTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAg < 1:465256/81‑1 (MQ=255)
gTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCt < 1:465257/80‑1 (MQ=255)
aCTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGaa > 1:465305/1‑81 (MQ=255)
gACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGaataa > 1:465308/1‑81 (MQ=255)
aCGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGaataat < 1:465258/81‑1 (MQ=255)
cTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCg > 2:465298/1‑81 (MQ=255)
cGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACTGAGAAGCTGAATAATCCGTCgg < 2:465259/79‑1 (MQ=255)
gTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGaa > 1:465313/1‑80 (MQ=255)
gTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAAt < 1:465260/81‑1 (MQ=255)
tCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATa < 2:465261/81‑1 (MQ=255)
ccGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGaa > 1:465309/1‑78 (MQ=255)
cGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTCACCGAGAAGCTGAATAATCCGTCGGAATa > 2:465312/1‑79 (MQ=255)
ggTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATagag < 2:465262/81‑1 (MQ=255)
cgcAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGt < 1:465263/81‑1 (MQ=255)
gcAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGt > 1:465301/1‑80 (MQ=255)
gcAACATCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTg < 2:465264/81‑1 (MQ=255)
aTCTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCg < 2:465265/81‑1 (MQ=255)
cTGATCGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGa > 1:465310/1‑80 (MQ=255)
cGGTGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCa > 1:465315/1‑81 (MQ=255)
tGCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgt < 2:465266/81‑1 (MQ=255)
gCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgtt > 2:465302/1‑81 (MQ=255)
gCTCGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgtt > 2:465328/1‑81 (MQ=255)
cGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTc > 1:465324/1‑81 (MQ=255)
cGATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTc > 2:465314/1‑81 (MQ=255)
gATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt < 2:465267/79‑1 (MQ=255)
gATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt < 2:465268/79‑1 (MQ=255)
gATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTcc < 1:465270/81‑1 (MQ=255)
gATGGCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTcc < 1:465269/81‑1 (MQ=255)
ggCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCAtc > 2:465306/1‑81 (MQ=255)
ggCGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCAtc > 2:465321/1‑81 (MQ=255)
cGTAGCGCTGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCAtcgt < 2:465271/81‑1 (MQ=255)
|
GGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGT > NC_000913/2707879‑2708028
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
