Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,836,333 |
G→A |
R365C (CGC→TGC) |
hypF ← |
carbamoyltransferase HypF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,836,333 | 0 | G | A | 100.0%
| 125.0
/ NA
| 36 | R365C (CGC→TGC) | hypF | carbamoyltransferase HypF |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/14); total (22/14) |
TTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAT > NC_000913/2836258‑2836407
|
ttttAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCAcacca > 1:488368/1‑81 (MQ=255)
ttAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACaccacc > 2:488373/1‑81 (MQ=255)
gCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatc < 2:488329/81‑1 (MQ=255)
gCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAat > 2:488363/1‑80 (MQ=255)
gCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAat > 1:488391/1‑80 (MQ=255)
cccGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatca > 1:488382/1‑81 (MQ=255)
cGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatcatc < 2:488330/81‑1 (MQ=255)
ggAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatcatc > 2:488376/1‑80 (MQ=255)
aGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATcatccat > 1:488372/1‑81 (MQ=255)
aaaGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGc > 1:488379/1‑81 (MQ=255)
aaaGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGc > 1:488390/1‑81 (MQ=255)
ccAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCa > 2:488371/1‑81 (MQ=255)
cAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCAc < 1:488331/81‑1 (MQ=255)
cAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCAc > 2:488358/1‑81 (MQ=255)
aGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCAc > 1:488384/1‑80 (MQ=255)
cgcATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGAt < 1:488332/81‑1 (MQ=255)
gcATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATg > 2:488386/1‑81 (MQ=255)
aTCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTc < 1:488333/81‑1 (MQ=255)
aTCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTc > 1:488369/1‑81 (MQ=255)
cGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTcgc < 1:488334/80‑1 (MQ=255)
ggCACATACCCCCGCGAACGGCGCAGCATTCCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCgg > 1:488380/1‑81 (MQ=255)
gCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGt < 1:488335/81‑1 (MQ=255)
cacaTACCCCCGCGAACGGCGCAGCATGTCGCCGCGTTCGCACAACACCGAATCATCCATCCGCTGCACGATGTCGCGGtt < 2:488336/81‑1 (MQ=255)
tACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATg > 2:488374/1‑80 (MQ=255)
ccGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATc > 2:488383/1‑79 (MQ=255)
ccGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCaa < 1:488337/81‑1 (MQ=255)
gcagcaTTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCgg < 2:488338/81‑1 (MQ=255)
gcagcaTTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCgg > 2:488375/1‑81 (MQ=255)
agcaTTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCa < 2:488339/81‑1 (MQ=255)
tCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGccc > 2:488394/1‑81 (MQ=255)
cgccgcTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGccc < 2:488340/80‑1 (MQ=255)
gccgcTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCt < 2:488341/80‑1 (MQ=255)
ccgcTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTg > 2:488399/1‑80 (MQ=255)
cTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCaaa > 1:488401/1‑81 (MQ=255)
tttCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAt > 1:488370/1‑81 (MQ=255)
tCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAt < 1:488342/79‑1 (MQ=255)
|
TTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAT > NC_000913/2836258‑2836407
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
