Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,879,257 |
C→T |
K172K (AAG→AAA) |
cas1 ← |
multifunctional nuclease Cas1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,879,257 | 0 | C | T | 76.5%
| 57.7
/ 16.9
| 34 | K172K (AAG→AAA) | cas1 | multifunctional nuclease Cas1 |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base T (16/10); total (20/14) |
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01 |
AGCTGCAAGTATCGCCGCTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCG > NC_000913/2879179‑2879326
|
aGCTGCAAGTATCGCCGCTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCttt > 2:497176/1‑81 (MQ=255)
tGCAAGTATCGCCGCTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTc > 1:497183/1‑81 (MQ=255)
aaGTATCGCCGCTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCa < 1:497149/81‑1 (MQ=255)
gccgcTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTc > 2:497181/1‑81 (MQ=255)
cTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTTGGAt > 2:497177/1‑81 (MQ=255)
cTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGAt < 1:497150/81‑1 (MQ=255)
ttCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATc > 2:497193/1‑81 (MQ=255)
cAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCg > 2:497178/1‑80 (MQ=255)
aGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCg > 1:497189/1‑79 (MQ=255)
aGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTa < 2:497151/81‑1 (MQ=255)
gTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAg < 2:497152/81‑1 (MQ=255)
ttACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGc < 2:497153/81‑1 (MQ=255)
aCGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCg < 1:497155/80‑1 (MQ=255)
aCGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCg < 1:497154/80‑1 (MQ=255)
cGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGAc > 1:497186/1‑81 (MQ=255)
gCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACg > 1:497199/1‑81 (MQ=255)
cGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGtc > 2:497180/1‑81 (MQ=255)
tataAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGtcca > 1:497182/1‑81 (MQ=255)
aaaCAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGtccattc > 2:497179/1‑81 (MQ=255)
aaaCAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGtccattc > 2:497202/1‑81 (MQ=255)
aCAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGACGtccattcca > 1:497184/1‑81 (MQ=255)
gcgcTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTAt < 1:497156/81‑1 (MQ=255)
gcTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTg < 1:497157/81‑1 (MQ=255)
cTAATGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGc < 1:497158/81‑1 (MQ=255)
aaTGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCtt > 2:497196/1‑81 (MQ=255)
aTGCATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTc > 1:497187/1‑81 (MQ=255)
cATTGGTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGc > 2:497190/1‑80 (MQ=255)
ggTTGATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGa < 1:497160/80‑1 (MQ=255)
ggTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGa < 1:497159/80‑1 (MQ=255)
ttGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGt < 2:497161/81‑1 (MQ=255)
gATCGTATCGCCTTTTTCCCAGTCTTTCGGCTCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGc > 1:497192/1‑81 (MQ=255)
gATCGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTg < 1:497162/80‑1 (MQ=255)
gATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGc > 2:497195/1‑81 (MQ=255)
cGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCg > 1:497188/1‑79 (MQ=255)
cGTATCGCCTTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCg > 2:497198/1‑79 (MQ=255)
|
AGCTGCAAGTATCGCCGCTTCAGTTACGCCGTATAAACAGGAAGTTGCAGCGCTAATGCATTGGTTGATCGTATCGCCCTTTTCCCAGTCTTTCGGATCGTAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCG > NC_000913/2879179‑2879326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
