Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,888,216 |
C→T |
A33T (GCT→ACT) |
cysH ← |
phosphoadenosine phosphosulfate reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,888,216 | 0 | C | T | 100.0%
| 105.6
/ NA
| 31 | A33T (GCT→ACT) | cysH | phosphoadenosine phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/7); total (24/7) |
CGCCTGAATGCCAAAGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGCGT > NC_000913/2888139‑2888293
|
cgccTGAATGCCAAAGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTc > 2:498405/1‑81 (MQ=255)
gccTGAATGCCAAAGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTc > 1:498407/1‑80 (MQ=255)
gAATGCCAAAGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGtt > 2:498410/1‑81 (MQ=255)
cAAAGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTc > 2:498396/1‑79 (MQ=255)
aaaGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAg > 1:498395/1‑81 (MQ=255)
cTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCg > 1:498408/1‑81 (MQ=255)
tGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCgg > 1:498399/1‑81 (MQ=255)
ggAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGc > 1:498424/1‑81 (MQ=255)
aaGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGt < 2:498369/81‑1 (MQ=255)
aaGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGt < 1:498368/81‑1 (MQ=255)
cATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTcc < 1:498370/80‑1 (MQ=255)
tatTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgc > 1:498403/1‑80 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgc > 1:498427/1‑79 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccag < 2:498371/81‑1 (MQ=255)
tCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagc > 1:498432/1‑81 (MQ=255)
aCCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgc > 2:498416/1‑81 (MQ=255)
cGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgc > 2:498417/1‑79 (MQ=255)
gggCAGATTATCCAGCGCCCAGGGTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgc > 2:498425/1‑78 (MQ=255)
ggCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag > 1:498402/1‑80 (MQ=255)
gCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGaa > 1:498404/1‑81 (MQ=255)
aGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATg > 1:498372/1‑81 (MQ=255)
aTCCAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATc > 2:498421/1‑81 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTAc > 1:498431/1‑81 (MQ=255)
gcgcCCAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCt > 2:498400/1‑81 (MQ=255)
ccAGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCgg > 2:498373/1‑81 (MQ=255)
aGGCTACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCgg > 2:498419/1‑79 (MQ=255)
tACGCGGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttc > 1:498414/1‑81 (MQ=255)
cgcgGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttc < 2:498374/79‑1 (MQ=255)
cgGCCTTCAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttcgttc < 1:498375/81‑1 (MQ=255)
cAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGc < 1:498376/79‑1 (MQ=255)
cAGTGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGCGt > 2:498420/1‑81 (MQ=255)
|
CGCCTGAATGCCAAAGCTGGAAGAAAGTACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGCGT > NC_000913/2888139‑2888293
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
