Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,890,712 |
T→C |
E396G (GAA→GGA) |
cysJ ← |
sulfite reductase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,890,712 | 0 | T | C | 100.0%
| 175.9
/ NA
| 50 | E396G (GAA→GGA) | cysJ | sulfite reductase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/22); total (28/22) |
AGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGC > NC_000913/2890635‑2890789
|
aGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCg < 2:498966/81‑1 (MQ=255)
ggCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGc < 2:498967/81‑1 (MQ=255)
caccacCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCt < 1:498968/81‑1 (MQ=255)
accaccGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTg > 2:499038/1‑81 (MQ=255)
caccGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGc > 2:499021/1‑80 (MQ=255)
ggCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCgag > 2:499027/1‑79 (MQ=255)
cACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGaggagg < 2:498969/81‑1 (MQ=255)
aCGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGc > 1:499025/1‑81 (MQ=255)
cGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCg < 2:498970/81‑1 (MQ=255)
gggCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGa > 1:499019/1‑81 (MQ=255)
gcgcGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATg < 1:498971/81‑1 (MQ=255)
ccACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGAc > 2:499037/1‑81 (MQ=255)
cACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGAcg > 2:499026/1‑81 (MQ=255)
gtcgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACgcgg > 2:498973/1‑81 (MQ=255)
gtcgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACgcgg < 2:498972/81‑1 (MQ=255)
tcgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc < 1:498974/81‑1 (MQ=255)
cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACgcggcg > 2:499042/1‑81 (MQ=255)
cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACgcggcg > 2:499031/1‑81 (MQ=255)
gtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACgcggcg < 1:498976/80‑1 (MQ=255)
gtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt > 1:499063/1‑81 (MQ=255)
gtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt < 1:498978/81‑1 (MQ=255)
gtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt < 2:498975/81‑1 (MQ=255)
gtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt < 2:498977/81‑1 (MQ=255)
aaCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt > 2:499029/1‑79 (MQ=255)
gCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCg > 2:499051/1‑81 (MQ=255)
ccacaccaACGGTGGCGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgc < 1:498979/81‑1 (MQ=255)
ccacaccaACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgc > 1:499035/1‑81 (MQ=255)
acaccaACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcgc < 2:498980/81‑1 (MQ=255)
acaccaACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcgc < 2:498981/81‑1 (MQ=255)
acaccaACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcgc < 2:498982/81‑1 (MQ=255)
accaACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag < 1:498983/81‑1 (MQ=255)
accaACGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag > 2:499039/1‑81 (MQ=255)
aaCGGTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag > 2:499034/1‑78 (MQ=255)
ggTGACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGatt < 2:498984/81‑1 (MQ=255)
gACGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaat > 1:499028/1‑81 (MQ=255)
cGTGTACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaat > 2:499047/1‑79 (MQ=255)
tgtACTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAgc < 1:498985/81‑1 (MQ=255)
cTTCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCtt > 2:499045/1‑81 (MQ=255)
tCGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCg > 1:499059/1‑81 (MQ=255)
cGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCgg > 1:499049/1‑81 (MQ=255)
cGTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCgg > 2:499057/1‑81 (MQ=255)
gTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCgg > 1:499056/1‑80 (MQ=255)
gTTCTCGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGc < 2:498986/81‑1 (MQ=255)
gTTCTCGACTCCCGCCTGCGAGGAGGCGATGAAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGc < 2:498987/81‑1 (MQ=255)
cGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATc > 1:499048/1‑79 (MQ=255)
cGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATc > 2:499052/1‑79 (MQ=255)
cGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATc > 2:499055/1‑79 (MQ=255)
cGACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATc > 1:499053/1‑79 (MQ=255)
gACTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCa < 1:498988/79‑1 (MQ=255)
aCTCCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGc > 1:499044/1‑81 (MQ=255)
|
AGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGC > NC_000913/2890635‑2890789
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
