Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,955,564 |
A→T |
L147Q (CTG→CAG) |
recB ← |
exodeoxyribonuclease V subunit RecB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,955,564 | 0 | A | T | 100.0%
| 125.9
/ NA
| 36 | L147Q (CTG→CAG) | recB | exodeoxyribonuclease V subunit RecB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (25/11); total (25/11) |
TAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTC > NC_000913/2955486‑2955637
|
tAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGc < 2:511355/81‑1 (MQ=255)
tAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGc < 2:511354/81‑1 (MQ=255)
gTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGcc < 2:511356/81‑1 (MQ=255)
gCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGa > 2:511398/1‑81 (MQ=255)
gCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCa > 1:511379/1‑80 (MQ=255)
aaTCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCAt > 1:511382/1‑81 (MQ=255)
cgcAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGt > 2:511417/1‑81 (MQ=255)
gcAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGtt < 1:511357/81‑1 (MQ=255)
gcAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGtt > 2:511397/1‑81 (MQ=255)
tGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATg > 1:511407/1‑81 (MQ=255)
agcgtagcAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCt > 2:511416/1‑81 (MQ=255)
agcgtagcAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCt > 1:511383/1‑81 (MQ=255)
agcgtagcAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCt > 2:511389/1‑81 (MQ=255)
cgtagcAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTgg > 2:511412/1‑81 (MQ=255)
agcAGAGACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCa > 1:511400/1‑80 (MQ=255)
agagACTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAg > 2:511424/1‑81 (MQ=255)
agaCTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGcc < 1:511358/81‑1 (MQ=255)
agaCTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGcc < 2:511359/81‑1 (MQ=255)
agaCTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGcc > 2:511387/1‑81 (MQ=255)
agaCTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGcc < 1:511360/81‑1 (MQ=255)
gaCTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCg > 1:511408/1‑81 (MQ=255)
aCTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGt > 1:511414/1‑81 (MQ=255)
cTCATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTg < 1:511361/81‑1 (MQ=255)
cATCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGaa > 2:511393/1‑81 (MQ=255)
tCTTCAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGaa > 1:511390/1‑79 (MQ=255)
cAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGt < 1:511362/79‑1 (MQ=255)
cAATCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTa > 2:511419/1‑80 (MQ=255)
aaTCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTaaa < 1:511363/81‑1 (MQ=255)
aaTCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTa > 2:511411/1‑79 (MQ=255)
aaTCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTa > 1:511388/1‑79 (MQ=255)
aTCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTa > 2:511409/1‑78 (MQ=255)
aTCAGCTGCTGCTCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTa > 1:511401/1‑78 (MQ=255)
tgctCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGGAAAAGCCGTGAATAGTAAAGACTGCCGCt > 2:511413/1‑81 (MQ=255)
tgctCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCt > 1:511420/1‑81 (MQ=255)
gctCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCtt > 2:511410/1‑81 (MQ=255)
ctCAAACTGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTc < 1:511364/81‑1 (MQ=255)
|
TAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGAGCATGCGCTGGCAAAAGCCGTGAATAGTAAAGACTGCCGCTTC > NC_000913/2955486‑2955637
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
