Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,958,495 |
A→G |
Y130Y (TAT→TAC) |
ptrA ← |
protease 3 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,958,495 | 0 | A | G | 100.0%
| 109.1
/ NA
| 30 | Y130Y (TAT→TAC) | ptrA | protease 3 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/12); total (18/12) |
AGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGATAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGACTGTC > NC_000913/2958424‑2958572
|
aGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGcc > 2:548587/1‑81 (MQ=255)
gCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCg > 1:548560/1‑81 (MQ=255)
cAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGt > 2:548571/1‑81 (MQ=255)
aGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGc > 1:548561/1‑80 (MQ=255)
aGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGc > 1:548565/1‑80 (MQ=255)
aGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCg < 1:548530/81‑1 (MQ=255)
aGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACgg > 2:548570/1‑81 (MQ=255)
aGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACg < 1:548531/80‑1 (MQ=255)
gCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGgc < 2:548532/81‑1 (MQ=255)
gCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTg > 2:548582/1‑81 (MQ=255)
aTCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCt > 1:548577/1‑81 (MQ=255)
ggCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGc < 1:548533/81‑1 (MQ=255)
cAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCAtt < 1:548534/81‑1 (MQ=255)
ggTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGAc > 2:548566/1‑81 (MQ=255)
tCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTa > 1:548581/1‑81 (MQ=255)
tACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAcc > 2:548579/1‑81 (MQ=255)
tACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAcc > 1:548575/1‑81 (MQ=255)
aCCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccg > 1:548580/1‑81 (MQ=255)
ccGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccgc < 1:548535/81‑1 (MQ=255)
cGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccgcc < 2:548536/81‑1 (MQ=255)
ggCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtt < 2:548537/81‑1 (MQ=255)
cAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATGCGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt > 1:548583/1‑81 (MQ=255)
aaGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt > 2:548584/1‑80 (MQ=255)
aaGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt > 2:548591/1‑80 (MQ=255)
gtcgtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGata > 2:548589/1‑81 (MQ=255)
tcgtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGatat < 2:548538/81‑1 (MQ=255)
gtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCATTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTc > 1:548576/1‑81 (MQ=255)
aaCTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGcc < 1:548539/79‑1 (MQ=255)
cTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGAc < 1:548540/81‑1 (MQ=255)
cAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGACTGTc < 2:548541/81‑1 (MQ=255)
|
AGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGATAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGACTGTC > NC_000913/2958424‑2958572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
