Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,960,676 |
C→T |
E585K (GAA→AAA) |
recC ← |
exodeoxyribonuclease V subunit RecC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,960,676 | 0 | C | T | 100.0%
| 149.6
/ NA
| 41 | E585K (GAA→AAA) | recC | exodeoxyribonuclease V subunit RecC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/19); total (22/19) |
GTCATCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAG > NC_000913/2960600‑2960751
|
gTCATCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGc > 1:549046/1‑81 (MQ=255)
aTCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGa < 2:549001/81‑1 (MQ=255)
cgccgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACg > 1:549043/1‑81 (MQ=255)
gccgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGc > 2:549051/1‑81 (MQ=255)
cgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTc < 2:549002/81‑1 (MQ=255)
cgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTc > 2:549041/1‑81 (MQ=255)
gcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTcc < 1:549003/81‑1 (MQ=255)
gcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTcc < 1:549004/81‑1 (MQ=255)
cTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCt < 2:549005/81‑1 (MQ=255)
cGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCtgtg > 1:549053/1‑81 (MQ=255)
ggTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGc > 2:549052/1‑81 (MQ=255)
ggTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGc > 1:549050/1‑81 (MQ=255)
cGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACg < 1:549006/81‑1 (MQ=255)
cAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGc > 2:549063/1‑79 (MQ=255)
aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg > 2:549062/1‑81 (MQ=255)
aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg < 2:549009/81‑1 (MQ=255)
aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg < 2:549007/81‑1 (MQ=255)
aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg < 2:549008/81‑1 (MQ=255)
gaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtt > 2:549056/1‑81 (MQ=255)
gaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtt > 1:549045/1‑81 (MQ=255)
gaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtc > 2:549047/1‑80 (MQ=255)
aagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGttt < 1:549010/81‑1 (MQ=255)
aagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtt > 1:549057/1‑80 (MQ=255)
tGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCa > 2:549064/1‑81 (MQ=255)
gCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCAt > 2:549058/1‑79 (MQ=255)
cATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAg > 2:549054/1‑81 (MQ=255)
atatCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAg > 1:549055/1‑80 (MQ=255)
atatCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGc < 2:549011/81‑1 (MQ=255)
tCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAg < 2:549012/80‑1 (MQ=255)
gcgACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTg < 2:549013/80‑1 (MQ=255)
cgACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTg > 2:549074/1‑79 (MQ=255)
aCAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc < 2:549014/81‑1 (MQ=255)
cAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc > 1:549049/1‑80 (MQ=255)
cAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc > 1:549061/1‑80 (MQ=255)
cAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc > 2:549073/1‑80 (MQ=255)
aCCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGa < 1:549015/81‑1 (MQ=255)
ggCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGc < 2:549016/81‑1 (MQ=255)
ggCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGc > 2:549078/1‑81 (MQ=255)
aCCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCcca < 2:549017/81‑1 (MQ=255)
cACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCccacc < 1:549018/81‑1 (MQ=255)
ctctTTCAGCGGACGCTCCTGTGCCAGCCCGCGACACCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAg < 1:549019/81‑1 (MQ=255)
|
GTCATCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAG > NC_000913/2960600‑2960751
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
