Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	524	31.4	1748232	97.8%	1709770	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,975,617	A→G	I133T (ATT→ACT)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,617	0	A	G	100.0%	126.7 / NA	36	I133T (ATT→ACT)	aas	acyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/21); total (15/21)

CGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAT  >  NC_000913/2975541‑2975693

cGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAAc                                                                          <  2:515495/81‑1 (MQ=255)

cGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAAc                                                                          >  2:515550/1‑81 (MQ=255)

        aGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCAc                                                                  >  2:515556/1‑81 (MQ=255)

           gACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCAc                                                                  >  2:515546/1‑78 (MQ=255)

                ttAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTc                                                          >  2:515528/1‑81 (MQ=255)

                 tAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTc                                                          <  1:515496/80‑1 (MQ=255)

                   aCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgcc                                                       >  2:515560/1‑81 (MQ=255)

                   aCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgc                                                        <  1:515497/80‑1 (MQ=255)

                   aCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgc                                                        <  1:515498/80‑1 (MQ=255)

                     cAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgccgc                                                     <  2:515499/81‑1 (MQ=255)

                       gACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGa                                                   >  1:515557/1‑81 (MQ=255)

                        aCCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGAc                                                  <  1:515500/81‑1 (MQ=255)

                         ccTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACa                                                 <  1:515501/81‑1 (MQ=255)

                             tCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACaaa                                               <  1:515502/79‑1 (MQ=255)

                              cAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAAcc                                            >  2:515561/1‑81 (MQ=255)

                              cAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAAcc                                            >  2:515562/1‑81 (MQ=255)

                               aGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAATCGTTGCACCAGACTTCGCCGCGACAAAAccc                                           >  2:515548/1‑81 (MQ=255)

                                ggcggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCcg                                          >  2:515521/1‑81 (MQ=255)

                                 gcggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCcgc                                         <  2:515503/81‑1 (MQ=255)

                                   ggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCgcgc                                       >  2:515549/1‑81 (MQ=255)

                                        aaGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATc                                   <  2:515504/80‑1 (MQ=255)

                                        aaGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCg                                  <  1:515505/81‑1 (MQ=255)

                                           tGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAg                               <  1:515506/81‑1 (MQ=255)

                                             cGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGa                              <  1:515507/80‑1 (MQ=255)

                                             cGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAt                             <  2:515508/81‑1 (MQ=255)

                                               tAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAttt                           <  1:515509/81‑1 (MQ=255)

                                                   ttCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTtcat                       <  2:515510/81‑1 (MQ=255)

                                                      cGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAg                    >  2:515565/1‑81 (MQ=255)

                                                        ccccTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCg                  <  1:515511/81‑1 (MQ=255)

                                                           cTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGc               <  2:515513/81‑1 (MQ=255)

                                                           cTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGc               <  1:515512/81‑1 (MQ=255)

                                                             tCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCt             >  1:515571/1‑81 (MQ=255)

                                                                    gCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtgg      >  2:515555/1‑81 (MQ=255)

                                                                    gCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtgg      >  2:515569/1‑81 (MQ=255)

                                                                        aGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAt  <  2:515515/81‑1 (MQ=255)

                                                                        aGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAt  <  1:515514/81‑1 (MQ=255)

CGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAT  >  NC_000913/2975541‑2975693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A