Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,034,269 |
T→C |
A302A (GCA→GCG) |
lysS ← |
lysine‑‑tRNA ligase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,034,269 | 0 | T | C | 100.0%
| 135.1
/ NA
| 38 | A302A (GCA→GCG) | lysS | lysine‑‑tRNA ligase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/24); total (14/24) |
GGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCA > NC_000913/3034191‑3034345
|
ggTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTgcgc > 2:525595/1‑81 (MQ=255)
gTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTgcgc > 1:525596/1‑80 (MQ=255)
aGTTTTTCGAACGGTTTACCGAAGTCCAGCATCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCag < 2:525550/80‑1 (MQ=255)
tttttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGt < 2:525551/81‑1 (MQ=255)
ttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACg > 2:525598/1‑81 (MQ=255)
gAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGaa < 2:525552/81‑1 (MQ=255)
cGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAg < 2:525553/81‑1 (MQ=255)
tACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGAtt > 1:525591/1‑81 (MQ=255)
tACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGAt < 1:525554/80‑1 (MQ=255)
aCCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc > 2:525636/1‑81 (MQ=255)
ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc < 1:525555/80‑1 (MQ=255)
ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc < 1:525556/80‑1 (MQ=255)
ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc < 1:525557/80‑1 (MQ=255)
aaGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGt > 2:525576/1‑80 (MQ=255)
aaGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTc < 1:525558/81‑1 (MQ=255)
cAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTc < 2:525559/81‑1 (MQ=255)
cAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTc < 1:525560/81‑1 (MQ=255)
aGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCg < 2:525561/81‑1 (MQ=255)
cGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt > 1:525614/1‑81 (MQ=255)
aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAg < 2:525562/80‑1 (MQ=255)
tCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt < 2:525563/81‑1 (MQ=255)
tCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt < 1:525564/81‑1 (MQ=255)
cGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt > 1:525602/1‑81 (MQ=255)
tAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa < 2:525565/81‑1 (MQ=255)
acctCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc < 2:525566/77‑1 (MQ=255)
ttCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGt > 2:525613/1‑80 (MQ=255)
ccGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa < 1:525567/80‑1 (MQ=255)
ccGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa < 1:525568/80‑1 (MQ=255)
cGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:525628/1‑81 (MQ=255)
gTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGcc < 2:525570/81‑1 (MQ=255)
gTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCG‑AAGCCa < 1:525569/81‑1 (MQ=255)
cTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt > 2:525606/1‑81 (MQ=255)
tACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 2:525604/1‑80 (MQ=255)
gagaATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:525618/1‑81 (MQ=255)
tatCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCca < 2:525571/81‑1 (MQ=255)
tatCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCca < 2:525572/81‑1 (MQ=255)
atCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat > 1:525600/1‑81 (MQ=255)
tCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 2:525573/80‑1 (MQ=255)
tCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat > 1:525610/1‑80 (MQ=255)
ccTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca > 2:525615/1‑81 (MQ=255)
|
GGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCA > NC_000913/3034191‑3034345
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
