Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,120,628 |
+T |
coding (652/1683 nt) |
glcA ← |
glycolate/lactate:H(+) symporter GlcA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,120,625 | 1 | . | T | 100.0%
| 114.8
/ NA
| 33 | G219R (GGG→AGG) | glcA | glycolate/lactate:H(+) symporter GlcA |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (21/12); total (21/12) |
TTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCC‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTG > NC_000913/3120552‑3120702
|
ttAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCC‑ttttc > 2:543105/1‑77 (MQ=255)
gagGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGc < 1:543054/80‑1 (MQ=255)
tAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTcc < 2:543055/81‑1 (MQ=255)
aGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatc < 2:543056/81‑1 (MQ=255)
gAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatca > 2:543093/1‑81 (MQ=255)
gAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatca < 2:543057/81‑1 (MQ=255)
aaCTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatcat > 1:543116/1‑81 (MQ=255)
tGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGc > 2:543099/1‑81 (MQ=255)
gAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGc > 1:543095/1‑80 (MQ=255)
gACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAc > 2:543112/1‑79 (MQ=255)
cAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagc < 1:543058/81‑1 (MQ=255)
gCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagcca < 2:543059/81‑1 (MQ=255)
gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagccag > 1:543101/1‑80 (MQ=255)
gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagccag > 2:543091/1‑80 (MQ=255)
gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagccag > 2:543082/1‑80 (MQ=255)
aaGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGaa < 2:543060/81‑1 (MQ=255)
gCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACg > 2:543110/1‑81 (MQ=255)
tCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGca > 2:543124/1‑81 (MQ=255)
tCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGca > 1:543089/1‑81 (MQ=255)
cccccAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGca > 2:543103/1‑80 (MQ=255)
cgccgcTGGCCACGTCTCTTTCACCCCTTTTCCATCCTTCCATCATTTCTACCATCCATATCGTCTCAAGAACCCACAgg > 1:857792/1‑80 (MQ=255)
gccgcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGaa > 1:543122/1‑81 (MQ=255)
ccgcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAAc > 2:543100/1‑81 (MQ=255)
gcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACgg > 1:543097/1‑81 (MQ=255)
gcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACgg < 1:543061/81‑1 (MQ=255)
gcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACgg > 1:543094/1‑81 (MQ=255)
ggCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTaa > 1:543086/1‑81 (MQ=255)
ggCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTaa > 2:543106/1‑81 (MQ=255)
ccACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACt < 2:543062/81‑1 (MQ=255)
cACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACt > 2:543090/1‑80 (MQ=255)
cACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTg < 2:543064/81‑1 (MQ=255)
cACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTg < 1:543063/81‑1 (MQ=255)
cGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGAc > 1:543121/1‑81 (MQ=255)
tCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGc < 1:543065/80‑1 (MQ=255)
acccct‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCAt > 2:543108/3‑81 (MQ=255)
cccct‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCAtt > 1:543128/2‑81 (MQ=255)
ccct‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTg > 1:543117/1‑81 (MQ=255)
|
TTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCC‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTG > NC_000913/3120552‑3120702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
