Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,122,870 |
T→C |
K312K (AAA→AAG) |
glcB ← |
malate synthase G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,122,870 | 0 | T | C | 100.0%
| 159.5
/ NA
| 44 | K312K (AAA→AAG) | glcB | malate synthase G |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/20); total (24/20) |
GATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGT > NC_000913/3122795‑3122946
|
gATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACg > 2:543530/1‑81 (MQ=255)
gATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACg < 2:543489/81‑1 (MQ=255)
aaCAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCAc < 2:543490/80‑1 (MQ=255)
aaCAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACg < 2:543491/81‑1 (MQ=255)
agcagcGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGAtt > 1:543539/1‑81 (MQ=255)
gcagcGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGAttt < 2:543492/81‑1 (MQ=255)
agcgagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGc > 2:543534/1‑81 (MQ=255)
agcgagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGc > 1:543529/1‑81 (MQ=255)
gcgagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGcc < 1:543493/81‑1 (MQ=255)
gcgagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGcc < 2:543494/81‑1 (MQ=255)
cgagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGcc > 2:543538/1‑80 (MQ=255)
gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGa > 2:543526/1‑81 (MQ=255)
gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGa > 1:543532/1‑81 (MQ=255)
gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGa > 2:543543/1‑81 (MQ=255)
gcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGAcc < 2:543495/81‑1 (MQ=255)
cgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCg < 1:543496/81‑1 (MQ=255)
gTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGt > 2:543522/1‑81 (MQ=255)
tCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGtt < 2:543497/81‑1 (MQ=255)
tCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGtt < 2:543498/81‑1 (MQ=255)
gTGCTGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGttttt > 1:543528/1‑81 (MQ=255)
gTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGttttt < 2:543499/81‑1 (MQ=255)
gTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGttttt > 1:543531/1‑81 (MQ=255)
cAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTtctc > 1:543541/1‑81 (MQ=255)
agaAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCAt < 1:543500/81‑1 (MQ=255)
gaAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCAtt > 1:543545/1‑81 (MQ=255)
aaTTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCAtttt < 2:543501/81‑1 (MQ=255)
cAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCtt > 1:543553/1‑81 (MQ=255)
gagCCATCGGCGGCGGTGTAATGACGAACGTCATTCAGCTTACGCACGATTTGCCGACCGTTTATCTTCATTTTCTCTTg > 1:543540/1‑80 (MQ=255)
agCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCa < 1:543502/81‑1 (MQ=255)
aTCGGCGGCGGTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGt > 2:543558/1‑81 (MQ=255)
ggcggcggTGTAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTccc > 2:543566/1‑81 (MQ=255)
tgtAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCAt > 1:543562/1‑79 (MQ=255)
tAATGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAg < 1:543503/80‑1 (MQ=255)
aaTGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGc > 1:543565/1‑81 (MQ=255)
aTGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGcc > 2:543542/1‑81 (MQ=255)
tGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGccc < 1:543505/81‑1 (MQ=255)
tGACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGccc < 1:543504/81‑1 (MQ=255)
gACGATCGTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGcc > 1:543564/1‑79 (MQ=255)
gTCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGtt > 1:543549/1‑81 (MQ=255)
tCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTg > 1:543544/1‑81 (MQ=255)
tCATTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTg > 2:543550/1‑81 (MQ=255)
aTTCAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGc < 1:543506/80‑1 (MQ=255)
cAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGt < 2:543507/80‑1 (MQ=255)
cAGCTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGt < 1:543508/80‑1 (MQ=255)
|
GATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGT > NC_000913/3122795‑3122946
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
